Biopolym. Cell. 1997; 13(5):408-415.
Gene-Engineering Biotechnology
A new retroviral vector system based on the duck-adapted variant of Pr-RSV-C with the extended host ranger
1Kaida I. V., 1Tsyba L. A., 1Boltovets P. N., 1Kashuba V. I.
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Abstract
We have constructed retroviral vector td da Pr-RSV-C e and helper virus td da Pr-RSV-C e' PS'Neo based on the duck-adapted variant of Pr-RSV-C with the extended host ranger and its transformation-defective mutant. To decrease the risk of transducing cell proto-oncogencs, E-element, supposedly involved in this process, was deleted. In order to obtain helper cell line the QT6 cells have been transfected by helper phismid p td da Pr-RSV-C e'PS~Neo. Packaging cells produce viral proteins, including p27gag, but intact helper virus is not detectable in reverse trans criptaxe assay.
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