Biopolym. Cell. 1997; 13(4):335-339.
http://dx.doi.org/10.7124/bc.000494
Viruses and Cell
Comparative studies of inhibitory action of synthetic, natural and complex preparations on HIV-1 clinical isolates obtained in Ukraine
1Antonenko S. V., 1Barbasheva O. V., 2Karpov A. V., 2Spivak N. Ya.
  1. Gromashevsky L.V. Institute of Epidemiology and Infection Diseases, AMS of Ukraine
    5, Amosova Str., Kyiv, Ukraine, 03038
  2. D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
    154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

In in vitro experiments using an acute HIV-infection model and clinical H1V isolates the authors have made comparative studies of modified nucleosides (AZT, D4T) protective action, the preparation being of different producers, as well as of polyribonucleic interferon inducers and of molecular complex yeast KNA – tilorone. It was found that, modified nucleosides producled in Ukraine do not significantly differ from foreign ones in their protective effect and their inhibitory action on p24-antigen synthesis, whereas interferon inducers is less effective, moreover yeast RNA – tilorone molecular complex acts better latest ones, and approach to nucleoside preparations according this effectivity. Taking into account the difference in the both type preparations mechanisms of action it was done conclusion about effectivity of their combined usage in AIDS therapy.
Full text: (PDF, in Ukrainian)

References

[1] Johnston MI, Hoth DF. Present status and future prospects for HIV therapies. Science. 1993;260(5112):1286-93.
[2] Shkaraputa L, Yazlovitsky A, Kononov O. Development of technology research and industrial production azidothymidine. I National Scientific Conference on HIV. AIDS (Kyiv, January 1995): Proc. ext. Kyiv, 1995:
[3] Poli G, Orenstein JM, Kinter A, Folks TM, Fauci AS. Interferon-alpha but not AZT suppresses HIV expression in chronically infected cell lines. Science. 1989;244(4904):575-7.
[4] Nosik NN, Nosik DN, Kuznetsova NV. [The inhibiting action of interferon inducers on the multiplication of the human immunodeficiency virus]. Vopr Virusol. 1992;37(2):92-4.
[5] Montefiori DC, Mitchell WM. Antiviral activity of mismatched double-stranded RNA against human immunodeficiency virus in vitro. Proc Natl Acad Sci U S A. 1987;84(9):2985-9.
[6] Krust B, Callebaut C, Hovanessian AG. Inhibition of entry of HIV into cells by poly(A).poly(U). AIDS Res Hum Retroviruses. 1993;9(11):1087-90.
[7] Karpov AV, Antonenko SV, Barbacheva YV, Spivak NYa, Cell genome stabilization by yeast RNA-tilorone complex, in in vitro HIV-infected cells. XI Int. Conf. on AIDS (Vancouver, July 7-12, 1996): Abstrs. Vancouver, 1996. Vol. II, Pub. A. 1022: 441.
[8] Karmanov EV, Lukashov VV. [Resistance to azidothymidine in human immunodeficiency virus (HIV) infection]. Mol Biol (Mosk). 1994;28(1):7-20. Review. Russian.
[9] Barbasheva OV, Antonenko SV, Grin OV. Improved methods of allocation of regional isolates of human immunodeficiency virus. Ukr. Nauk. Med. Molodezh. Zh. 1995:(2):30-2.
[10] Chandra P, Zunino F, G?tz A. Bis-deae-fluorenone: A specific inhibitor of DNA polymerases from RNA tumor viruses. FEBS Lett. 1972;22(2):161-164.
[11] Karpov OV, Zholobak NM. Interferonogenic properties of yeast RNA - tilorone complex. Dopovidi Nats Akad Nauk Ukrainy. 1996; (3):128-31.