Biopolym. Cell. 1997; 13(3):239-244.
http://dx.doi.org/10.7124/bc.000485
Viruses and Cell
Detection of classical swine fever virus by reverse transcriptase-polymerase chain reaction and molecular hybridization
1Kirilenko S. D., 2Deriabin O. M., 2Deriabina O. G., 1Kirilenko O. L., 1Cherednik Ju. O., 2Grlshok L. P., 2Shikov O. T.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of Veterinary Medicine, NAAS of Ukraine
    30, Donetska Str., Kyiv, Ukraine, 03151

Abstract

Oligonucleotide primers flanking 846 bp fragment of gp5l-55 structural gene of classical swine fever virus (CSFV) have been used in polymerase chain reaction for detection of genomic RNA of CSFV in pathological specimens taken from animals with different clinical signs and disease running. The possibility of their use in the differentiation of high- and low virulent CSFV isolates and in molecular epidemiologicat investigations is discussed.
Full text: (PDF, in Ukrainian)

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