Biopolym. Cell. 1997; 13(3):232-238.
Molecular Mechanisms of Differentiation
Optimization of the system for emhryogenetic studies on inbred strains of mice. 3. Conditions of hormonal stimulation of females and in vitro culture of preimplanlation embryos of ICR, CBA/Lac and C57Bl/6j mouse strains
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Optimal conditions for hormonal stimulation of females of ICR, CBAlLac and C57Bl/6j have bean worked out The optimal concentration and spectrum of hormones for each strain was determined. Conditions for in vitro culture of zygotes of different strains were elaborated. It was determined that the important thing for successive in vitro culture of zygotes is the stage of development of fertilized ova, which is characterized by the time of appearance of maternal and paternal pronuclei.
 Gates AH. Methods in mammalian embryology. Ed. J. Daniel. San Francisco: Freeman Co., 1971: 64-75.
 Dyban AP. Experiments on embryos of mammals. Methods for Developmental Biology. Ed. BL Astaurov. M.: Nauka, 1974: 220-1.
 Monk M. Mammalian Development: A Practical Approach: Oxford University Press. 1987; 340 p.
 Stolina MR, Morozova LM, Solomko AP. Optimization of the system for genetic-embryological studies of inbred mice strains. 1. Comparison of in vitro fertilization ability of inbred strain mice BALB/cLac, C57BL/6j and ICR. Biopolym Cell. 1992; 8(2):49-52.
 Morozova LM, Stolina MR, Solomko AP. Optimization of the system for genetic-embryological studies of inbred mice strains. 2. Comparative characterization of season female fertility of BALB/cLac, C57BL/6j and ICR mice. Biopolym Cell. 1992; 8(3):33-6.
 Whitten WK. Nutrient requirements for the culture of preimplantation embryons in vitro. Adv Biosci. 1971;6: 129-39.
 Hogan B, Constantini F, Lacy E. Manipulating the mouse embryo. A laboratory manual. New York : Cold Spring Harbor Lab., 1986; 350 p.
 Spearow JL. Characterization of genetic differences in hormone-induced ovulation rate in mice. Reproduction. 1988;82(2):799вЂ“806.
 Golubitsa AN, Amstislavskiy SYa, Fayzulin RZ. et al. Super ovulatory response in different strains of mice in response to the behavior of gonadotropin preparations. Problems of preservation and maintenance of genetic collections labora tory animals. Conservation of genetic resources: Proc. scientific. tr. Pushchino, 1991: 93-97.
 Abramczuk J, Solter D, Koprowski H. The beneficial effect EDTA on development of mouse one-cell embryos in chemically defined medium. Dev Biol. 1977;61(2):378-83.
 Noniashvili EL, Dyban AP. Parthenogenetical mice oocytes development induced by ethanol and by heat shock in vitro and in situ. General patterns and control. mechanisms of early embryogenesis of mammals in health and disease. Leningrad: Nauka, 1985:22-30.
 Luthardt FW, Donahue RP. Pronuclear DNA synthesis in mouse eggs. An autoradiographic study. Exp Cell Res. 1973;82(1):143-51.
 Krishna M, Generoso WM. Timing of sperm penetration, pronuclear formation, pronuclear DNA synthesis, and first cleavage in naturally ovulated mouse eggs. J Exp Zool. 1977;202(2):245-52.
 Howlett SK, Bolton VN. Sequence and regulation of morphological and molecular events during the first cell cycle of mouse embryogenesis. J Embryol Exp Morphol. 1985;87:175-206.
 Kaufman MH, Sachs L. Complete preimplantation development in culture of parthenogenetic mouse embryos. J Embryol Exp Morphol. 1976;35(1):179-90.
 Graham CF, Lehtonen E. Formation and consequences of cell patterns in preimplantation mouse development. J Embryol Exp Morphol. 1979;49:277-94.
 Goddard MJ, Pratt HP. Control of events during early cleavage of the mouse embryo: an analysis of the '2-cell block'. J Embryol Exp Morphol. 1983;73:111-33.
 Dyban AP. Mammals early development. L.: Nauka, 1988; 228 p.
 Gulyas BJ, Wood M, Whittingham DG. Fusion of oocytes and development of oocyte fusion products in the mouse. Dev Biol. 1984;101(1):246-50.
 Muggleton-Harris A, Whittingham DG, Wilson L. Cytoplasmic control of preimplantation development in vitro in the mouse. Nature. 1982;299(5882):460-2.
 Pratt HP, Muggleton-Harris AL. Cycling cytoplasmic factors that promote mitosis in the cultured 2-cell mouse embryo. Development. 1988;104(1):115-20.
 Van Blerkom J. Protein synthesis during oogenesis. Biology of Fertilization. Eds A. Monroy, C. Metz. New York, 1983: 289-320.
 Bolton VN, Oades PJ, Johnson MH. The relationship between cleavage, DNA replication, and gene expression in the mouse 2-cell embryo. J Embryol Exp Morphol. 1984;79:139-63.
 Johnson MH, McConnell J, Van Blerkom J. Programmed development in the mouse embryo. J Embryol Exp Morphol. 1984;83 Suppl:197-231.
 Chatot CL, Ziomek CA, Bavister BD, Lewis JL, Torres I. An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro. J Reprod Fertil. 1989;86(2):679-88.
 Lawitts JA, Biggers JD. Optimization of mouse embryo culture media using simplex methods. J Reprod Fertil. 1991;91(2):543-56.
 Lawitts JA, Biggers JD. Overcoming the 2-cell block by modifying standard components in a mouse embryo culture medium. Biol Reprod. 1991;45(2):245-51.