Biopolym. Cell. 1997; 13(3):209-212.
http://dx.doi.org/10.7124/bc.000480
Structure and Function of Biopolymers
Comparative analysis of histone HI and HI0 structure and properties
1Protas A. F.
  1. National University of Kyiv-Mohyla Academy
    2, Skovorody Str., Kyiv, Ukraine, 04655

Abstract

The properties of histones HI and HI0 from rat brain cortex free in solution and in chromatin were studied. Histones HI and HI0 have the constants for quenching of own fluorescence with acrylamide 1,4 M and 2,0 M–1 and the binding constants Kias with 1,8-ANS 14,6 MM u 5,5 MM. Histone HI0 forms high-molecular weight aggregates in solution with NaCl cristals very poorly as well as polymers in chromatin. It is supposed that histone HI0 function in chromatin is realised on a local site.
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References

[1] Terpilovskaya ON, Ivanov VA. Structural and functional organization of chromatin in mammals nerve cells. Usp Sovrem Biol. 1990; 110(1(4)):118-33.
[2] Thoma F, Koller T. Unravelled nucleosomes, nucleosome beads and higher order structures of chromatin: influence of non-histone components and histone H1. J Mol Biol. 1981;149(4):709-33.
[3] Nikolaev LG, Glotov BO, Dashkevich VK, Barbashov SF, Severin ES. [Localization of histone H1 in chromatin. Cross-linking of the N- and C-terminal halves of the molecule with bifunctional reagents]. Mol Biol (Mosk). 1984;18(3):736-42.
[4] Khrapunov SN, Protas AF, Sivolob AV, Dragan AI, Berdyshev GD. Intrinsic fluorescence, difference spectrophotometry and theoretical studies on tertiary structure of calf thymus histone H1. Int J Biochem. 1985;17(2):217-22.
[5] Protas AF, Khrapunov SN, Berdyshev GD. [Interaction of histone H1 molecules in a solution]. Ukr Biokhim Zh. 1986;58(5):22-6.
[6] Pehrson J, Cole RD. Histone H10 accumulates in growth-inhibited cultured cells. Nature. 1980;285(5759):43-4.
[7] Medvedev ZhA, Medvedeva MN. High H/H1 histone ratio in spontaneous hepatomas in ageing mice. IRCS Med. Sci. 1980; 8: 431.
[8] Protas AF. Effect of low dose radiation on the structure and function of chromatin of cells of the cerebral cortex. Radiobiol. Congress (Kyiv, September 20-25, 1993): Proc. rep. Kiev, 1993: 840-841.
[9] Osadchaya MM. Methods of biochemical research. L.: Meditsina, 1982: 36-43.
[10] Panyim S, Chalkley R. High resolution acrylamide gel electrophoresis of histones. Arch Biochem Biophys. 1969;130(1):337-46.
[11] Protas AF, Chaialo PP. [The effect of low doses of external gamma irradiation on the chromatin structure and activity of the histone-specific proteinases of rat brain cells]. Radiobiologiia. 1991;31(5):733-8.
[12] Ashapkin VV, Vanyushin BF. Differential gene activity in the brains of animals and biochemical mechanisms of its regulation. Usp Sovrem Biol. 1984; 98(3(6)):323-37.