Biopolym. Cell. 1997; 13(1):86-88.
http://dx.doi.org/10.7124/bc.00046D
Viruses and Cell
Comparative analysis of immunology specific C-terminus region of cDNA for the capsid protein of the two new TVM strains
1Stepanyuk S. A.
  1. Taras Shevchenko National University of Kyiv
    64, Volodymyrska Str., Kyiv, Ukraine, 01033

Abstract

Two strains of TVM – TVM NT9 and TVM LE7 have been isolated from plants in regions with 137Cs nuclear contamination on apparently 12.6 and 11 Ci/km2. Recombinant plasmids (pTVM9, pTVM9.5t pTVM7t pTVM7.5) containing cDNA of full genome and cDNA for C-end specific capsid protein region, correspond from each strain of isolated viruses, have been obtained. Sequencing analysis of capsid C-terminus cDNA of pTVM9.5 and pTVM7.5 revealed two conservative amino acid replacements at position 130, 148 for TVM NT9 strain and one nonconservative amino acid replacement at position 148 for TVM LE7 strain, which probably don't influence immunospecificity of isolated strains
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References

[1] Kuzin AM. On the variability of the main molecular mechanisms under heavy and weak y-radiation influence on organism. Izv Akad Nauk SSSR. 1980. 6: 883-890.
[2] Boyko AL. Ecology of plant viruses. K.: Vyshch sk, 1990; 167 p.
[3] Sugiyama T, Korant BD, Lonberg-Holm KK. RNA virus gene expression and its control. Annu Rev Microbiol. 1972;26:467-502.
[4] Fundamental techniques in virology. Eds Habel K. Salzman NP. 1969 Academic press New York London.
[5] Noordam D. Identification of plant viruses. Methods and experiments. Ed. Noordam D. Wageningen: Pudoc, 1973. 277 p.
[6] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.
[7] Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265-75.
[8] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.
[9] DNA cloning: a practical approach. Ed. DM Glover. Oxford, IRL Press, 1985; Vol. 1 and 2: 435 p
[10] Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977;74(12):5463-7.
[11] Fraenkel-Conrat H. The Chemistry and Biology of Viruses. Academic Press. 1969; 294 p.
[12] Boyko AL, Stepanujk SA, Garifulin OM. Sequencing analysis cDNA for C-terminus part of capsid protein for tomato TVM isolated from region with nuclear contamination. Biopolym Cell. 1996; 12(5):100-5.
[13] Boyko AL, Stepanjuk SA, Garifulin OM. Obtaining and analysis of provirus cDNA for C-terminus part of capsid protein for tobacco TVM strain isolated from region with nuclear contamination. Biopolym Cell. 1996; 12(6):112-5.
[14] Radavsky YuV. Antigenic structure of the core proteins of plant viruses and protein antigens Bortetella pertussis: Auth Thesis. ... dr biol. nauk. Kiev, 1994. 32 p.
[15] Dayhoff MO, Schwartz RM, Orcutt BC. In Atlas of protein sequence and structure. Ed. M. O. Dayhoff. New York: Natl. Biomed. Res. Found, 1979. 352 p.