Biopolym. Cell. 1997; 13(1):18-21.
http://dx.doi.org/10.7124/bc.000461
Structure and Function of Biopolymers
The use of polyamines for creation of effective reconstructed protein synthesizing system from rabbit liver
1Palchevskii S. S.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

A cell-free reconstructed protein-synthesizing system from rabbit liver has been developed. The system contains highly purified homologous components of translation apparatus and is characterized by a sufficient rate of polypeptide synthesis. The use of spermine and spermidine has allowed to optimize the activity of the system at physiological concentration of Mg2+.
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