Biopolym. Cell. 1996; 12(6):112-115.
http://dx.doi.org/10.7124/bc.00045D
Obtaining and analysis of provirus cDNA for C-terminus part of capsid protein for tobacco TVM strain isolated from region with nuclear contamination
1Boyko A. L., 1Stepanjuk S. A., 2Garifulin O. M.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Taras Shevchenko National University of Kyiv
    64, Volodymyrska Str., Kyiv, Ukraine, 01033

Abstract

Two identical strains of tobacco type TVM have been isolated at region with Cs nuclear contamination with apparently 12,6 Ci/km2 activity density and recombinant plasmids (pTVMQ, pTVM9.5) with insert ofcDNA provirus and cDNAfor C-end specific capsid protein region correspondency from one of isolated viruses have been obtained. Sequencing analysis of cDNA pTVM9.5 revealed on one nonconservative amino acid replacement of leucine instead of valine in position 130 with comparison of that at standard TVM sequencing.
Full text: (PDF, in Russian)

References

[1] Sasson A. Biotechnologies: challenges and promises. UNESCO 1985
[2] Boyko AL. Ecology of plant viruses. K.: Vyshch sk, 1990; 167 p.
[3] Fundamental techniques in virology. Eds Habel K. Salzman NP. 1969 Academic press New York London.
[4] Noordam D. Identification of plant viruses. Methods and experiments. Wageningen: Pudoc., 1973. 277 p.
[5] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.
[6] Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265-75.
[7] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.
[8] DNA cloning: a practical approach. Ed. DM Glover. Oxford, IRL Press, 1985; Vol. 1 and 2: 435 p
[9] Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977;74(12):5463-7.
[10] Fraenkel-Conrat H. The Chemistry and Biology of Viruses. Academic Press. 1969; 294 p.
[11] Dayhoff MO, Schwartz RM, Orcutt BC. In Atlas of Protein Sequence and Structure. Ed. M. O. Dayhoff. Natl. Biomed. Res. Found, 1979: 345-52.