Biopolym. Cell. 1996; 12(6):69-73.
Characterization of catalytic activity in vitro of the ribozyme targeted to tat-RNA of HIV-I
1Buryanovsky L. N., 1Shved A. D.
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Abstract
Essential properties of catalytic activity in vitro of the anti-tat RNA (HIV-I) hammerhead ribozyme have been studied. Spermidine-dependencies, discovered by us earlier, has supported. Optimal concentration of magnesium ion were established and was equal 5–10 mM. Temperature optimum of reaction was 30 °C. 20 % formamide has stimulating influence on efficiency of the cleavage at 15 °C. Time-dependence course of efficiency of reaction also were carried out.
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