Biopolym. Cell. 1996; 12(6):69-73.
Characterization of catalytic activity in vitro of the ribozyme targeted to tat-RNA of HIV-I
1Buryanovsky L. N., 1Shved A. D.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Essential properties of catalytic activity in vitro of the anti-tat RNA (HIV-I) hammerhead ribozyme have been studied. Spermidine-dependencies, discovered by us earlier, has supported. Optimal concentration of magnesium ion were established and was equal 5–10 mM. Temperature optimum of reaction was 30 °C. 20 % formamide has stimulating influence on efficiency of the cleavage at 15 °C. Time-dependence course of efficiency of reaction also were carried out.