Biopolym. Cell. 1996; 12(5):29-33.
http://dx.doi.org/10.7124/bc.000445
DNA probe construction with the use of silver salt. 3. Dosed modification of two-stranded probe and additional colouring of hybrids
1Shugalii A. V., 1Lichina M. V.
  1. Institute of Chemical Physics, RAS in Chernogolovka
    Academician Semenov avenue 1, Chernogolovka, Moscow region, Russian Federation, 142432

Abstract

The different ways of the coloured probe preparation with the use of the metal complex comprising ethanol-amine-formaldehyde chains forming coordination bonds with silver ions have been discussed. The denaturated DNA. partially unwind with formaldehyde molecules, and the probe-template duplexes have been modified on the basis of the branching of oligomer chains already formed. The sensitivity of the detection of 10 % -modified Off A immobilized on nitrocellulose filters has reached dozens of picogramms.
Full text: (PDF, in Russian)

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