Biopolym. Cell. 1996; 12(4):73-83.
Lyzogeny by MS2 phage. Expression of MS2-specific information with segregants of unstable transducing P1- and λ phages
1Pererva T. P., 1Miriuta A. J., 1Woodmaska M. I., 2Alekseenko I. P.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of Veterinary Medicine, NAAS of Ukraine
    30, Donetska Str., Kyiv, Ukraine, 03151

Abstract

Using genetical methods the group of phages segregating in succession from each other was obtained. In one case these phages originate from Pi-like transducing plasmid and in another case – from transducing λ-phage mutant (λ-4), integrated DNA-fragment of E. coli MS2-induced mutant. Serological properties of final phage variants demonstrate MS2 phage genetical information expression and appear to be evidence of its presence in the structure of all precedent phage forms.

References

[1] Pererva TP. Resistance to phage MS2 induced in E. coli by infection with that phage. Tsitol Genet. 1977;11(1):3-9.
[2] Pererva TP, Malyuta SS. The system of E. coli MS2-induced mutants on F-factor. Molek Biol (Kiev). 1984; Iss 38:81-90.
[3] Pererva TP, Miryuta NYu, Miryuta AYu. MS2 phage lysogeny. Phage-specific RNA synthesis on cell DNA. Biopolym Cell. 1993; 9(1):45-50.
[4] Pererva TP, Mirjuta NYu, Mirjuta AYu, Woodmaska MI, Zherebtsova EN. Lysogeny by MS2 phage. Analysis of a recombinant plasmid containing MS2 RNA-like sequence. Biopolym Cell. 1995; 11(1):61-5.
[5] Zyskind JW, Smith DW. DNA replication, the bacterial cell cycle, and cell growth. Cell. 1992;69(1):5-8.
[6] Asai T, Kogoma T. D-loops and R-loops: alternative mechanisms for the initiation of chromosome replication in Escherichia coli. J Bacteriol. 1994;176(7):1807-12.
[7] Adams M. Bacteriophages. New York; London: im. Publ. Inc., 1951.
[8] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.
[9] Davis RW, Simon M, Davidson N. Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Nucleic Acids, Part D. 1971;413–28.
[10] Luria SE, Adams JN, Ting RC. Transduction of lactose-utilizing ability among strains of E. coli and S. dysenteriae and the properties of the transducing phage particles. Virology. 1960;12:348-90.
[11] Pererva TP, Buch IG, Martynov SA, Malyuta SS. Transfer attributes of impaired growth, division and functional properties of membranes and membrane of Escherichia coli using other strains P1-like plasmids and transducing phages lambda and P1. Molek Biol (Kiev). 1984. Iss 38:90-5.
[12] Lane D, Hill D, Caughey P, Gunn P. The mini-F primary origin. Sequence analysis and multiple activities. J Mol Biol. 1984;180(2):267-82.
[13] Pererva TP, Buch IG, Danilenko TS, Martynov SA, A-4 phage transducing properties. Viruses and virus diseases. 1988. Iss. 16: 46-9.
[14] Daniels DI, Schroeder JI, Szybalski W, Blathner FR. A molecular map of bacteriophage lambda. Genetic Maps. 1982. 2: 1-9.
[15] Pickett GG, Peabody DS. Encapsidation of heterologous RNAs by bacteriophage MS2 coat protein. Nucleic Acids Res. 1993;21(19):4621-6.