Biopolym. Cell. 1996; 12(4):31-37.
Development of potentiometric immunosensor for interferon detection
1Sergeyeva T. A., 1Soldatkin A. P., 1Rachkov A. E., 1Tereschenko M. I., 1Piletsky S. A., 1El'skaya A. V.
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Abstract
The effective immunosensor for α2-interferon detection based on ion-sensitive field effect transistor has been developed. A specific sensingelement is fabricated by immobilizing interferon on the gate of pH-sensitive field effect transistor (pH-FET). The interaction of anti-interferon antibodies labelled with β-lactamase with. interferon-pH-FET (in the presence of specific enzyme substrate) leads to a local pH-change and produces electrochemical signal which is in proportion to the conjugate concentration. The main working characteristics of the sensor obtained have been estimated. The competitive electroimmunoassay has been employed for interferon detection in analysed solution. The sensor linear response to interferon concentration is obtained in the range 10–200 (μg/mi This gives a possibility of interferon detection in nondiluted cultural medium. The data of the competitive electroimmunoassay are in a good accordance with the ELISA ones. The possibility of regeneration of the immunosensor obtained has been shown.
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