Biopolym. Cell. 1996; 12(4):31-37.
http://dx.doi.org/10.7124/bc.000437
Development of potentiometric immunosensor for interferon detection
1Sergeyeva T. A., 1Soldatkin A. P., 1Rachkov A. E., 1Tereschenko M. I., 1Piletsky S. A., 1El'skaya A. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The effective immunosensor for α2-interferon detection based on ion-sensitive field effect transistor has been developed. A specific sensingelement is fabricated by immobilizing interferon on the gate of pH-sensitive field effect transistor (pH-FET). The interaction of anti-interferon antibodies labelled with β-lactamase with. interferon-pH-FET (in the presence of specific enzyme substrate) leads to a local pH-change and produces electrochemical signal which is in proportion to the conjugate concentration. The main working characteristics of the sensor obtained have been estimated. The competitive electroimmunoassay has been employed for interferon detection in analysed solution. The sensor linear response to interferon concentration is obtained in the range 10–200 (μg/mi This gives a possibility of interferon detection in nondiluted cultural medium. The data of the competitive electroimmunoassay are in a good accordance with the ELISA ones. The possibility of regeneration of the immunosensor obtained has been shown.
Full text: (PDF, in Russian)

References

[1] Intron A recommended by EC committee. Appl Genet News. 1991. 11(12):6.
[2] Wong R, Morejon A, Ivonne OR et al. Treatment with human leukocyte interferon versus recombinant alpha 2 interferon in Cuban patients with combined infection HBsAG. YIH. Interferon Biotechnologia: Symp. 1, Int. Conf. Ctr. (Apr. 17-22, 1989, Havana). Havana, 1989: SOI-001.
[3] Ludwig CU. Possibilities and limits of the use of interferons in the clinic. Schweiz Med Wochenschr. 1989;119(44):1539-43.
[4] Pilyavskaya EA, Baboshko YA. Two-site enzyme-linked immunosorbent test system for the determination of alpha-interferon. Proc. Conf. on the 85th anniversary of Tomsk. Research Institute of Vaccines and Sera NGO "Virion". Tomsk, 1991; 4(2):40-2.
[5] Novlev VI, Stepanov AN. Quantitative determination of interferon using an immunoenzyme analysis method. Tr Inst Im Pastera. 1988;64:108-13.
[6] Solov'yeva IYe, Verbov VYa, Kondrat'yeva GA. Development of enzyme immunoassay for human alpha interferon testing. Genetically engineered human alpha interferon. L., 1988: 101-5.
[7] Boubriak OA, Soldatkin AP, Starodub NF, Sandrovsky AK, El’skaya AK. Determination of urea in blood serum by a urease biosensor based on an ion-sensitive field-effect transistor.Sens Actuators B Chem. 1995;27(1-3):429–31.
[8] State Pharmacopoeia of the USSR. M.: Meditsina, 1968: 980-2.