Biopolym. Cell. 1996; 12(3):101-103.
The simple method for cloning of human embryonic hemopoietic cells
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Abstract
We have modified a method for cloning of human embryonic hemopoietic cells. This method is based on providing of natural growth and differentiation factors and preservation of native organ hemopoietic micromedia
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References
[1]
Chervenick PA, LoBuglio AF. Human blood monocytes: stimulators of granulocyte and mononuclear colony formation in vitro. Science. 1972;178(4057):164-6.
[2]
Knudtzon S, Mortensen BT. Growth stimulation of human bone marrow cells in agar culture by vascular cells. Blood. 1975;46(6):937-43.
[3]
Galbraith PR, Baker FL, Cooke LJ, Morley DC Jr, Sinclair J, Parker S, Brisbin D. Factors influencing in vitro production of colony-stimulating factor by mononuclear leukocytes from humans. Can Med Assoc J. 1979;121(2):172-8.
[4]
Afanas'yev BV, Almazov VA. Parental human hematopoietic cells. Physiology and pathology. L.: Nauka, 1985. 204 p.
[5]
Bradley TR, Hodson GS, Bertoncello I. Characteristics of primitive macrophage progenitor cells with high proliferative potential: Relationship to cells with marrow repopulating ability in 5-fluorouracil treated mouse bone marrow. Exp hematol today. Eds S. J. Baum et al. Basel, 1980: 285-97.
[6]
Paran M, Sachs L, Barak Y, Resnitzky P. In vitro induction of granulocyte differentiation in hematopoietic cells from leukemic and non-leukemic patients. Proc Natl Acad Sci U S A. 1970;67(3):1542-9.
[7]
Fojo SS, Wu MC, Gross MA, Purcell Y, Yunis AA. Purification and characterization of a colony stimulating factor from human lung. Biochemistry. 1978;17(15):3109-16.
[8]
Bliumkin VN, Skaletski? NN, Babikova RA, Kirsanova LA, Zagrebina OV. Preparation of liver cultures from human embryos. Biull Eksp Biol Med. 1988;105(2):212-4.
[9]
Grishchenko VI, Lobyntseva GS, Votyakova IA, Shereshnikov SI. Hematopoietic cells of fetal liver (embryogenesis, cryopreservation and transplantation). Kiev: Naukova Dumka, 1988;192 p.
[10]
Friedenstein AJ, Luria EA. Cellular basis of hematopoietic microenvironment. M: Meditsina. 1980. 216 p.
[11]
Fridenshte?n AIa. Osteogenic stem cells of the bone marrow. Ontogenez. 1991;22(2):189-97.