Biopolym. Cell. 1996; 12(3):91-96.
http://dx.doi.org/10.7124/bc.000432
Participation of calcium ions in the process of mycelial dimorphism of Theielavia terrestris
1Gromozova E. N., 1Blazhchuk I. S.
  1. D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
    154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

Calcium ions are the intermediates between signals of external medium and cells' reaction. The influence of different salts of calcium on the morphogenesis of T. terrestris was studied. It was shown that different concentrations of CaCl2, CaSO4, CaCO3, Ca(NO3)2 and Ca(CH3COO)2 didn't change diffuse form of fungus. However, 15 mM of CaCCl2 and 10 mM of CaSO4 and higher concentrations of these salts switched over pellets growth to the diffuse one. The process was observed after CaCl2 adding lag-phase of growth being sensitive to morphogenic signal. Morphogenic action of calcium ions was confirmed by the experiments with usingof veropamil. On the basis of obtained with chlorpromasin data the calmodulin participation in this process is discussed. The calcium ions effect by means ofadenylate system on morphogenetical program of T. terrestris development is supposed.
Full text: (PDF, in Russian)

References

[1] Rekabi AL, Sijal A. Effect of calcium vegetative growth and sporangia production of Achlya prolifera Nees. J Biol Sci Res. 1987; 18(3): 69-74.
[2] Cooper-Palomar JL, Powell MJ. Sites of calcium concentration during sexual reproduction of Achlya ambisexualis. Mycologia. 1988;80(6):783-9.
[3] O'Day DH, Lydan MA. The regulation of membrane fusion during sexual development in Dictyostelium discoideum. Biochem Cell Biol. 1989;67(7):321-6.
[4] St Leger RJ, Butt TM, Staples RC, Roberts DW. Second messenger involvement in differentiation of the entomopathogenic fungus Metarhizium anisopliae. J Gen Microbiol. 1990;136(9):1779–89.
[5] Takeuchi Y, Schmid J, Caldwell JH, Harold FM. Transcellular ion currents and extension of Neurospora crassa hyphae. J Membr Biol. 1988;101(1):33-41.
[6] Martinez-Cadena G, Ruiz-Herrera J. Activation of chitin synthetase from Phycomyces blakesleeanus by calcium and calmodulin. Arch Microbiol. 1987;148(4):280–5.
[7] Ruiz-Herrera J, Valenzuela C, Martinez-Cadena G, Obregon A. Alterations in the vesicular pattern and wall growth of Phycomyces induced by the calcium ionophore A 23187. Protoplasma. 1989;148(1):15–25.
[8] Brunton AH, Gadd GM. Evidence for an inositol lipid signal pathway in the yeast-mycelium transition of Ophiostoma ulmi, the Dutch elm disease fungus. Mycological Research. 1991;95(4):484–91.
[9] Paranjape V, Roy BG, Datta A. Involvement of calcium, calmodulin and protein phosphorylation in morphogenesis of Candida albicans. J Gen Microbiol. 1990;136(11):2149-54.
[10] Muthukumar G, Nickerson KW. Ca(II)-calmodulin regulation of fungal dimorphism in Ceratocystis ulmi. J Bacteriol. 1984;159(1):390-2.
[11] Clarke M, Bazari WL, Kayman SC. Isolation and properties of calmodulin from Dictyostelium discoideum. J Bacteriol. 1980;141(1):397-400.
[12] Gromozova EN, Blazhchuk IS. Effect of certain factors on the Thielavia sp. Growth pattern in the submerged culture. Mikrobiol Zh. 1989; 51(4):30-1.
[13] Shemshur TV, Gromozova EN, Podgorsky VS. Effect of certaincultivation conditions on the character of the ascomycetes growth in a submerged culture. Mikrobiol Zh. 1989; 51(3):30-3.
[14] Gromozova ON, Blazhchuk IS, Podgorsky VS. Studies pool adenosine 3'-5'-monophosphate in the process of the mycelium structures Thielavia terrestris in submerged conditions. Ukr Bot Zh. 1995; 52(5).
[15] Blumberg DD, Comer JF, Walton EM. Ca++ antagonists distinguish different requirements for cAMP-mediated gene expression in the cellular slime mold, Dictyostelium discoideum. Differentiation. 1989;41(1):14-21.
[16] Sokolovsky VY, Philippovich SY, Afanasyeva TP et al. Effect Ca2 + on the activity of some enzymes involved in the regulation of carotenogenesis in Neurospora crassa. Prikl Biokhim Mikrobiol. 1987; 28(1): 71-7.