Biopolym. Cell. 1996; 12(3):47-53.
http://dx.doi.org/10.7124/bc.00042C
The activity of antioxidant enzymes and free-radical processes in neurones cells nucleus under low-dose irradiation
1Protas A. F.
  1. Ukrainian Research Center for Radiation Medicine, Academy of Medical Sciences of Ukraine
    53, Melnikova Str., Kyiv, Ukraine, 04050

Abstract

The external gamma-irradiation of rats under 0.1–1 Gy alters the activity of nuclear superoxide dismutase (SOD), catalase (CAT), glutathione-peroxidase (GP) and glutathione-reductase (GR). The activity of SOD is maximal at the first two days after irradiation. Irradiation not alters the summal CAT and GP activity. The activity of GR decreased just after irradiation, but it is restored in 5 days. This process is correlated with decreasing of the reduced glutathione. The increasing of stabile free-radical productes is observed. It has some dependence on dose of irradiation. The model analysis of purified CAT, GP and GR showed, that unde exposure with free radicals the activities of these enzymes are decreased.
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References

[1] Vartanian LS, Rashba IuE, Nagler LG, Zgurski? AA, Oktiabr'skaia LA, Bilenko MV. Membranes of subcellular organelles as the source of superoxide radicals in liver ischemia. Biull Eksp Biol Med. 1990;109(6):550-2.
[2] Del Maestro R, McDonald W. Subcellular localization of superoxide dismutases, glutathione peroxidase and catalase in developing rat cerebral cortex. Mech Ageing Dev. 1989;48(1):15-31.
[3] Protas AF, Chaialo PP. Superoxide dismutase in the nuclei of cerebral cortex cells of rats: isolation, properties, effect of ionizing radiation. Ukr Biokhim Zh. 1993;65(2):73-8.
[4] S?len G, Edgren M, Scott OC, R?v?sz L. Cellular glutathione content and K values. Int J Radiat Biol Relat Stud Phys Chem Med. 1987;51(1):39-44. PubMed
[5] S?len G, Edgren M, Scott OC, R?v?sz L. Cellular glutathione content and K values. II. Int J Radiat Biol. 1989;55(2):201-10.
[6] Emerit I. Reactive oxygen species, chromosome mutation, and cancer: possible role of clastogenic factors in carcinogenesis. Free Radic Biol Med. 1994;16(1):99-109.
[7] Wynter CV. Persistence of altered RNA synthesis in rat cerebral cortex 12 h after a single electroconvulsive shock. J Neurochem. 1979;32(2):495-504.
[8] Koroliuk MA, Ivanova LI, Ma?orova IG, Tokarev VE. A method of determining catalase activity. Lab Delo. 1988;(1):16-9.
[9] G?nzler WA, Kremers H, Floh? L. An improved coupled test procedure for glutathione peroxidase (EC 1-11-1-9-) in blood. Z Klin Chem Klin Biochem. 1974;12(10):444-8.
[10] Methods of biochemical studies. Ed. M I. Porokhova L. Publ Leningr univers, 1982. 272 p.
[11] Torchinskiy YuM. Sulfur in the proteins. M.: Nauka, 1977. 302 p.
[12] Modern Methods in Biochemistry. Ed. BN. Orekhovicha. M.: Meditsina, 1977. 68 p.
[13] Protas AF, Chaialo PP. The effect of low doses of external gamma irradiation on the chromatin structure and activity of the histone-specific proteinases of rat brain cells. Radiobiologiia. 1991;31(5):733-8.
[14] Baraboy VA, Orel VE, Karnaukh YAM. Peroxidation and radiation. Kiev Naukova Dumka. 1991; 256 p.
[15] Vladimirov YuA, Archakov AI. Lipid peroxidation in biological membranes. Moscow: Nauka, 1972; 252 p.
[16] Lardinois OM. Reactions of bovine liver catalase with superoxide radicals and hydrogen peroxide. Free Radic Res. 1995;22(3):251-74.
[17] Gubsky YuI, Levitsky EL. Mechanisms of the lipid peroxidation of the liver rats chromatin fractions. Biopolym Cell. 1993; 9(5):34-43.
[18] Levytsky? EL, Gubsky? IuY. Free radical damage of the nuclear genetic apparatus of cells. Ukr Biokhim Zh. 1994;66(4):18-30.
[19] Free radicals in molecular biology, aging and desease. Ed. by D. Armstrong. New York: Raven press, 1984. Vol. 27. 540 p.
[20] Giulivi C, Boveris A, Cadenas E. Hydroxyl radical generation during mitochondrial electron transfer and the formation of 8-hydroxydesoxyguanosine in mitochondrial DNA. Arch Biochem Biophys. 1995;316(2):909-16.
[21] Superoxide and superoxide dismutase in chemistry, biology and medicine. Ed. by G. Rotilio. Amsterdam; New York; Oxford: Elsevier, 1986. 688 p.
[22] Chaialo PP, Protas AF. Isoenzyme spectrum of lactate dehydrogenase, malate dehydrogenase, esterase and acid phosphatase of rat brain cells at different times after external 1 Gy gamma irradiation. Radiobiologiia. 1992;32(6):815-9.