Biopolym. Cell. 1996; 12(2):68-73.
Genome and Its Regulation
Approaches to gene identification of Campylobacteria genus bacteria by polymerase chain reaction with universal primers
1Kirik D. L., 2Burianovsky L. N., 1Sliablovskaya E. A., 1Pinchuc I. V., 1Krolevetskaya N. M., 1Kikot V. I.
  1. Gromashevsky L.V. Institute of Epidemiology and Infection Diseases, AMS of Ukraine
    5, Amosova Str., Kyiv, Ukraine, 03038
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Preliminary studies on development of gene identification method of Campylobacteria genus bacteria on the base of polymerase chain reaction (PCR) with using universialoligonucleotide primers – REP (repetitive extragenic palindromic sequences). For all strains of Campylobacter jejuni PCR-product was peculiar 600 pairs ofnucleotides. Five group ofPCR-serovars in strains C. jejuni, one serovar (Lio 32) differed on minoric fragments. It can be of importance for difinition of infection source while epidemic analysis.

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