Biopolym. Cell. 1996; 12(2):56-61.
http://dx.doi.org/10.7124/bc.000422
Cell Biology
Composition of cellular walls in callus cultures of strawberries with different capacity to morphogenesis
1Yablokova E. V., 2Kiyamova R. G., 1Tuleneva N. M., 2Kolesnichenko E. V., 1Gorshkova T. A., 2Zheltonozhskaya L. V.
  1. Kazan Institute of Biology, RAS
    Lobachevsky street, 2/31, Kazan, Russian Federation, 420111
  2. National Agrarian University UAAN
    15, Heroyev Oborony Str., Kyiv, Ukraine, 03041

Abstract

The composition of polysaccharides and phenol combinations of cell walls in callus cultures of strawberries with different ability to regeneration of the plant has been investigated. No essential differences have been found either in the content of cell walls or in the content and composition of poly saccharide fractions. At the same time the spectrum of phenol compounds had some clear-cut distinction: among the phenol products of oxidation of cell walls in regenerating callus cultures the vanilinaccountedfor a considerable fraction which was totally missing in nonregenerating calluses. Spectrophotometric analysis has made itpoissible to suppose that this phenomenon involves the presence offerulic acid in the cell walls of regenerating cultures.
Full text: (PDF, in English)

References

[1] Fry SC. The growing plant cell wall: Chemical and metabolic analysis. Ed. M. Wiikins. New York: John Willey and Sons, 1988. 333 p.
[2] Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant. 1962;15(3):473–97.
[3] Updegraff DM. Semimicro determination of cellulose in biological materials. Anal Biochem. 1969;32(3):420-4.
[4] Hartley RD. Improved methods for the estimation by gas-liquid chromatography of lignin degradation products from plants. J Chromatogr A. 1971;54:335–44.
[5] Willemse MTA, Emons AMC. Autofluorescence and HPLC analysis of phenoilcs in Zea mays L. stem cell wall. Acta bot neer. 1991; 40(2): 115-24.
[6] Harris PJ, Hartley RD. Detection of bound ferulic acid in cell walls of the Gramineae by ultraviolet fluorescence microscopy. Nature.; 1976;259(5543):508–10.
[7] Kato Y, Yamanouchi H, Hinata K, Ohsumi C, Hayashi T. Involvement of Phenolic Esters in Cell Aggregation of Suspension-Cultured Rice Cells. Plant Physiol. 1994;104(1):147-152.
[8] Locher R, Martin HV, Grison R, Pilet P-E. Cell wall-bound trans- and cis-ferulic acids in growing maize roots. Physiol Plant. 1994;90(4):734–8.