Biopolym. Cell. 1996; 12(2):42-46.
http://dx.doi.org/10.7124/bc.000420
Cell Biology
Genetic transformation of potato (Solanum tuberosum L.) using a binary Agrobacterium tumefaciens vector with patatin promoter class I
1Kovalenko P. G., 1Yefimenko I. M., 1Schuman N. V., 1Medvedeva T. V., 2Gazaryan K. G., 1Galkin A. P.
  1. Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine
    1, Murmans'ka Str., Kyiv, Ukraine, 02094
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Kanamycin resistant plants of S. tuberosum L. (in vitro-grown) cv. Zarevo were obtained from tlie cocultivated microtubers with A. tumefaciens. A disarmed binary vector systems containing the neomycin phosphotransferase (NPTII) gene as selectable marker and chloramphenicol acetyltransferase (CAT), as a reporter gene, under control of new patatin promoter class I were utilized. In vitro grown minitubers discs were used as sources of explants to produce transgenic plants on selective medium containing 100 fig/1 kanamycin and CAT enzyme activities were detected.
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References

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