Biopolym. Cell. 1996; 12(2):29-33.
Structure and Function of Biopolymers
Hydroxamic derivatives of saturated fatty acids as inhibitors of 5-lipoxygenase
1Bondarenko L. B., 1Ohiy S. A., 1Tsernjuk V. N., 1Butovich I. A.
  1. Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine
    1, Murmans'ka Str., Kyiv, Ukraine, 02094

Abstract

In experiments in vitro were established inhibitory effects ofhydroxamic derivatives ofstearic, palmitic and caprilic acids on 5-lipoxygenase from potato tubers. Their effects depended on pli and substrate concentrations and were independent – on time of incubation of enzyme with inhibitor. Hydroxamic derivative of stearic acid was the most effective inhibitor among investigated compounds. Sliortening of fatty acid side chain lead to sharp decreasing of inhibitory effect.

References

[1] Rukuch J. Leukotrienes and lipoxygenases. Amsterdam. Elsevier, 1989. 518 p.
[2] Butovich IA, Kharchenko OV, Bondarenko LB, Babenko VM, Livarchuk LV. Linoleyl-hydroxamic acid: a 5-lipoxygenase inhibitor. Biokhimiia. 1994; 59(6):808-12.
[3] Butovich IA, Kharchenko OV, Kukhar VP, Moibenko AA. Kinetic scheme inhibition of lipoxygenase from porcine leukocytes by linoleyl-hydroxamic acid. Abstr. 1st Int. Congr. of ISSFAL. Lugano; Milan, 1993: 94.
[4] Kukhar VP, Butovich IA. Synthesis and study of prostaglandins. Proc. Dokl. IV All-Union. Conf. Minsk, 1989: 110.
[5] Holman PT, Bergstrom F. The Enzymes. Ed. B. Sumner. New York; London: Acad, press, 1954. Vol. 2, pt 1: 559-80.
[6] Butovich IA, Parshikova TV, Babenko VM. et al. Regulatory role of phospholipids in the oxidation of linoleic acid by 5-lipoxygenase. Biol Membrane. 1992; 9(6):611-6.
[7] Keleti T. Basic Enzyme Kinetics. Akad?miai Kiad?, 1986. 420 p.
[8] Butovich IA, Kukhar' VP. Potato 5-lipoxygenase. Kinetics of linoleic acid oxidation. Ukr Biokhim Zh. 1989;61(2):106-8.