Mapping of Bacillus subtilis riboflavin operon fragment coding for 3,4-dihydroxy-2-butanone-4-phosphate synthase

Boretska, N. I.; Liauta-Teglivets, O. Ye.; Voronovsky, A. Y.; Boretsky, J. R.; Shavlovsky, G. M.

doi:10.7124/bc.000414

Biopolym. Cell. 1996; 12(1):64-68.

http://dx.doi.org/10.7124/bc.000414

Mapping of Bacillus subtilis riboflavin operon fragment coding for 3,4-dihydroxy-2-butanone-4-phosphate synthase

1Boretska N. I., 1Liauta-Teglivets O. Ye., 1Voronovsky A. Y., 1Boretsky J. R., 1Shavlovsky G. M.

Division of Cell Regulatory Systems of O.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine
14/16, Drahomanov Str., Lviv, Ukraine, 79005

Abstract

Complementation analysis of Bacillus subtilis riboflavin operon was carried out by means of Escherichia coli riboflavin deficient strains and recombinant plasmids. It was found that ORF-3 ofB. subtilis riboflavin operon encodes for a bifunctional enzyme. It catalyses formation of both 2, 5-diamino-4-hydroxy-6-ribosylamino-pyrimidine-5'-phosphate from GTP and 3, 4-dihydroxy-2-butanone-4-phosphate from ribuhse-5-phosphate.

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References

[1] Mironov VN, Kraev AS, Chernov BK, Ul'ianov AV, Golova IuB. [Riboflavin biosynthesis genes of Bacillus subtilis--complete primary structure and organization model]. Dokl Akad Nauk SSSR. 1989;305(2):482-7. Russian.

[2] Boretski? IuR, Drobinskaia IE, Batchikova NV, Bidnenko VE, Rabinovich PM. [Subcloning and study of the GTP-cyclohydrolase gene of Bacillus subtilis]. Mol Gen Mikrobiol Virusol. 1991;(7):22-5. Russian.

[3] Richter G, Volk R, Krieger C, Lahm HW, R?thlisberger U, Bacher A. Biosynthesis of riboflavin: cloning, sequencing, and expression of the gene coding for 3,4-dihydroxy-2-butanone 4-phosphate synthase of Escherichia coli. J Bacteriol. 1992;174(12):4050-6.

[4] Bandrin SV, Rabinovich PM, Stepanov AI. [3 linkage groups of the genes of riboflavin biosynthesis in Escherichia coli]. Genetika. 1983;19(9):1419-25. Russian.

[5] Shavlovski? GM, Tesliar GE, Strugovshchikova LP. [Flavinogenesis regulation in riboflavin-dependent Escherichia coli mutants]. Mikrobiologiia. 1982;51(6):986-92. Russian.

[6] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.

[7] O'Kane DJ, Karle VA, Lee J. Purification of lumazine proteins from Photobacterium leiognathi and Photobacterium phosphoreum: bioluminescence properties. Biochemistry. 1985;24(6):1461-7.

[8] O'Kane DJ, Woodward B, Lee J, Prasher DC. Borrowed proteins in bacterial bioluminescence. Proc Natl Acad Sci U S A. 1991;88(4):1100-4.

[9] Bacher A, Mail?nder B. Biosynthesis of riboflavin in Bacillus subtilis: function and genetic control of the riboflavin synthase complex. J Bacteriol. 1978;134(2):476-82.

[10] Richter G, Ritz H, Katzenmeier G, Volk R, Kohnle A, Lottspeich F, Allendorf D, Bacher A. Biosynthesis of riboflavin: cloning, sequencing, mapping, and expression of the gene coding for GTP cyclohydrolase II in Escherichia coli. J Bacteriol. 1993;175(13):4045-51.

[11] Mironov NN. Bacillus subtilis riboflavin biosynthesis genes, the complete nucleotide sequence and operon organization: Author. dis. ... kand biol nauk. M .: IMB AN SSR 1989; 20 p.

[12] Boretski? IuR, Skoblov IuS, Khodova OM, Rabinovich PM. [Purification and properties of GTP-cyclohydrolase from Bacillus subtilis]. Biokhimiia. 1992;57(7):1021-30. Russian.

[13] Shavlovski? GM, Logvinenko EM. [Supersynthesis of flavins in microorganisms and its molecular mechanism (review of the literature)]. Prikl Biokhim Mikrobiol. 1988;24(4):435-47. Review. Russian.