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Organ-specific gene expression in transgenic potato: the cloning a new promoter of a class I patatin gene
- Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine
1, Murmans'ka Str., Kyiv, Ukraine, 02094
- Institute of Molecular Genetics, Academy of Sciences of the USSR
2, Academician Kurchatov Sq., Moscow, Russian Federation, 123182
Using synthetic oligonucleotide probes homologous to conservative AT-rich motif of patatin genes class I of two different clones were isolated from a potato genomic library. One of two different genomic clones named λpat122 was subcloned and analysis 5'-region sequences. Using the chloramphenicol acetyltransferase (CAT) gene as a reporter it has been shown that a 1.8 kb promoter fragment of the class I patatin gene λpat122 provides all the information necessary for both tuber-specific and sucrose-induced expression in leaves in transgenic potato plants.
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