Biopolym. Cell. 1995; 11(6):75-80.
Interrelations of the Klebsiella genera with the plant. 2. Localization of K. oxytoca and K. terriaena into the tobacco and wheat tissues
1Petak A. M., 1Kovtunovich G. L., 1Kozyrovskaya N. A., 1Turyanitsa A. I., 1Kordyum V. A.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Bacteria K. oxytoca 13183,VN13, M5al-47, K. terrigena 80-07, tagged with the bioluminescence genes (lux) of Photobacteruim leiognathi, are able to be localized inside tissue of tobacco and wheat in both leaves and roots. There were revealed 10 fold less bacteria isolated from inner tissues as compared to bacteria isolated from the plant surface. The loss of the lux genes in bacteria developing in the plant tissue less 1 % that is considerably lower than in bacteria growing in the continuous culture. Location of JC oxytoca VN13 and K. terrigena 80-07 inside the wheat roots has been proved by electron microscopy analysis. The artificial association of Nicotiana tobacco – K. oxytoca VN13 preserved the associative interactions under transition of bacteria in a cycle leaf – seedling → leaf – seedling.

References

[1] Brill WJ. Biochemical genetics of nitrogen fixation. Microbiol Rev. 1980;44(3):449-67.
[2] Kozyrovskaya N. A., Makitruk V. L., Ruckdashell E. Nitrogen-fixing Klebsiella species produce indole-3-acetic acid. Biopolym Cell. 1990; 6(6):93-96.
[3] Nguyen T. N. H., Ton T. N. B., Tarasenko V. A., Kozyrovskaja N. A. Nitrogen-fixing bacteria colonize rice root xylema. Biopolym Cell. 1989; 5(2):97-99
[4] Kozyrovska N, Alexeyev M, Kovtunovych G. et al. Survival of Klebsiella oxytoca VN13 engineered to bioluminescence on barley roots during plant vegetation. Microbial Releas. 1994; 2:261-265.
[5] Alexeyev M. F., Gunkovskaya N. V. Method for rapid transformation of enterobacteria and some factors influencing its efficiency. Biopolym. Cell. 1992;8(3):46-50.
[6] Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant. 1962;15(3):473–97.
[7] Kao KN, Michayluk MR. A method for high-frequency intergeneric fusion of plant protoplasts. Planta. 1974;115(4):355-67.
[8] Belyavskaya N. O., Kozyrovskaya N. O., Kucherenko L. O., Kordyum E. L., Kordyum V. A. Interrelations of the Klebsiella genera with the plant. I. Electron microscopic analysis of endophytic microorganisms interrelationship with rice seedling roots. Biopolym Cell. 1995;11(1):55-60
[9] Leshak AM, Turyanitsa AN, Kozyrovskaya NA. Study of influence of bacteria of the genus Klebsiella on regenerative processes. Fiziologiya i biokhimiya kul'turnykh rasteniy. 1995; 5
[10] Reinhold B, Hurek T, Fendrik I. Cross-reaction of predominant nitrogen-fixing bacteria with enveloped, round bodies in the root interior of kallar grass. Appl Environ Microbiol. 1987;53(4):889-91.
[11] Dobereiner J, Reis VM, Lazarin AC. New N2-fixing bacteria in association with cereals and sugar cane. Nitrogen fixation: hundred years after. Eds H. Bothe, F. J. De Bruijn, W. E. Neton. Stuttgart: Gustav Fisher, 1988: 717-722.
[12] Dobereiner J., Reis V. M.r Paula M. A., Olivares F. Endophytic diazotropfs in sugar cane, cereals and tuber plants. New horizons in nitrogen fixation. Eds R. Palacios, J. Mora, W. E. Newton. Dordrecht: Kluwer Acad. Publ., 1993: 671-676.
[13] You C, Zhou F. Non-nodular endorhizospheric nitrogen fixation in wetland rice. Can J Microbiol. 1989;35(3):403–8.