Biopolym. Cell. 1995; 11(6):69-74.
The effects of myocardial ischemia on the aggregate state of rabbit liver aminoacyl-tRNA synthetases and tRNA methyltransferases
- Kaunas Medical Academy
Mizkevicha Str. 9, Kaunas, Lithuania, 44307
- Vilnius University, Institute of Biochemistry
12, Mokslininku Str., Vilnius, Lithuania, 08662
The activities of aminoacyl-lRNA synthetases and tRNA methyltransferases in postribosomal extracts from normal rabbit liver and 12 hr after experimental myocardial ischemia (EMI), as well as their distribution between high-molecular-mass complexes and free enzymes fractions, have been compared. An increase in the activity of glutamyl-, glycyl-, leucyl-, lysyl- and seryl-tRNA synthetases by 12–44 % has been determined under EMI. The total tRNA methyltransferase activity is increased up to 47 %. In the case of EMI among the methylated products of submethylated tRNA the contents of m1A, m5C, m 5U, O-m-R are decreased, and the content of m7G is increased Gel chromatography of the postribosomal extract on a Sephadex G-200 column shows that aminoacyl-tRNA synthetase and tRNA-methyltransferase activities are distributed between the high-molecular-mass complex (Mr > 1·106 Daltons) and fractions of lower molecular mass complexes and free enzymes. In the of EMI aminoacyl-tRNA synthetase and tRNA methyltransferase activiteis are partly redistributed from the high-molecular-mass complex into th elowel molecular mass fraction, what may be related to the alteration of protein biosynthesis in liver under myocardial ischemia
 Osterman AA. The participation of tRNA in the regulation of protein synthesis at the translational level in eukaryotes. Usp biol khimii. 1980; 21(6):54-78.
 El'skaya A. V., Starodub N. F., Potapov A. P., Kovalenko M. I., Ovcharenko G. V., Obolenskaya M. U., Ivanov L. L. Regulation of protein biosynthesis in eukaryotes Kiev: Nauk. dumka, 1990 280 p.
 Mirande M. Aminoacyl-tRNA synthetase family from prokaryotes and eukaryotes: structural domains and their implications. Prog Nucleic Acid Res Mol Biol. 1991;40:95-142.
 Saxholm HJ, Pitot HC. Characterization of a proteolipid complex of aminoacyl-tRNA synthetases and transfer RNA from rat liver. Biochim Biophys Acta. 1979;562(3):386-99.
 Vellekamp GJ, Kull FJ. Allotropism in aspartyl-tRNA synthetase from procine thyroid. Eur J Biochem. 1981;118(2):261-9.
 Harris CL, Marin K, Stewart D. tRNA sulfurtransferase: a member of the aminoacyl-tRNA synthetase complex in rat liver. Biochem Biophys Res Commun. 1977;79(3):657-62.
 Iaremchuk AD, Tarasiavichene LE, Kondratiuk TP, El'skaia AV. Aminoacyl-tRNA-synthetases and their high molecular weight complexes in the regenerating rat liver. Mol Biol (Mosk). 1984;18(5):1336-41. Russian.
 Agris PF, Playl T, Goldman L, Horton E, Woolverton D, Setzer D, Rodi C. Processing of tRNA is accomplished by a high-molecular-weight enzyme complex. Recent Results Cancer Res. 1983;84:237-54.
 Agris PF, Setzer D, Gehrke CW. Characterization of a unique enzyme complex composed of S-adenosyl-L-methionine-tRNA-methyltransferase and aminoacyl-tRNA synthetase activities. Nucleic Acids Res. 1977;4(11):3803-19.
 Ryazanov AG. Does the complex of aminoacyl-tRNA synthetases and tRNA-modifying enzymes prevent miscoding? FEBS Lett. 1984;178(1):6-9.
 Prashkevichius A., Maskoliunas R., Stapulionis R. et al. Protein biosynthesis machinery under myocardial ischemia. Exp Biol. 1990,(1)47-55.
 Lekis AV, Mashanauskas TK, Ivanov LL et al. Effect of cultured Polyscias cells on the activity of components of the protein-synthesizing system of rabbit liver. Khimiko-farmakologicheskiy zhurnal. 1988; 22(8):970-973.
 Toleikis A, Dzeja P, Praskevicius A, Jasaitis A. Mitochondrial functions in ischemic myocardium. I. Proton electrochemical gradient, inner membrane permeability, calcium transport and oxidative phosphorylation in isolated mitochondria. J Mol Cell Cardiol. 1979;11(1):57-76.
 Ivanov LL, Lukoshiavichius LIu, Kovalenko MI, Bagdonaite OD, Lekis AV. Amino acyl tRNA synthetase complexes in rabbit liver in experimental myocardial infarction. Ukr Biokhim Zh. 1983;55(4):368-71.
 Lukoshiavichius LIu, Rodovichius GA, Kovalenko MM, Pivoriuna?te II, Prashkiavichius AK. tRNA and aminoacyl-tRNA synthetases from the liver of rabbits in experimental myocardial infarction. Vopr Med Khim. 1983;29(4):65-9.
 Bjork GR, Svensson I. Analysis of methylated constituents from RNA by thin-layer chromatography. Biochim Biophys Acta. 1967;138(2):430-2.
 Ivanov LL, Tamuliavichius AA, Lukoshiavichius LIu, Kovalenko MI, Rodovichius GA. Aminoacyl-tRNA-synthetases and their high molecular weight complexes in experimental myocardial ischemia. Mol Biol (Mosk). 1984;18(5):1326-9.
 Ivanov LL, Martinkus Z, Kharchenko OV, Sara S, Lukoshevichius L, Prashkevichius A, El'skaya AV. Subcellular distribution and properties of rabbit liver aminoacyl-tRNA synthetases under myocardial ischemia. Mol Cell Biochem. 1993;125(2):105-14.