Biopolym. Cell. 1995; 11(6):69-74.
The effects of myocardial ischemia on the aggregate state of rabbit liver aminoacyl-tRNA synthetases and tRNA methyltransferases
1Viezeliene D. V., 1Taraseviciene L. E., 1Ivanov L. L., 1Jasaitis A. A., 1Lukosevicius L. J.
  1. Kaunas Medical Academy
    Mizkevicha Str. 9, Kaunas, Lithuania, 44307
  2. Vilnius University, Institute of Biochemistry
    12, Mokslininku Str., Vilnius, Lithuania, 08662

Abstract

The activities of aminoacyl-lRNA synthetases and tRNA methyltransferases in postribosomal extracts from normal rabbit liver and 12 hr after experimental myocardial ischemia (EMI), as well as their distribution between high-molecular-mass complexes and free enzymes fractions, have been compared. An increase in the activity of glutamyl-, glycyl-, leucyl-, lysyl- and seryl-tRNA synthetases by 12–44 % has been determined under EMI. The total tRNA methyltransferase activity is increased up to 47 %. In the case of EMI among the methylated products of submethylated tRNA the contents of m1A, m5C, m 5U, O-m-R are decreased, and the content of m7G is increased Gel chromatography of the postribosomal extract on a Sephadex G-200 column shows that aminoacyl-tRNA synthetase and tRNA-methyltransferase activities are distributed between the high-molecular-mass complex (Mr > 1·106 Daltons) and fractions of lower molecular mass complexes and free enzymes. In the of EMI aminoacyl-tRNA synthetase and tRNA methyltransferase activiteis are partly redistributed from the high-molecular-mass complex into th elowel molecular mass fraction, what may be related to the alteration of protein biosynthesis in liver under myocardial ischemia

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