Biopolym. Cell. 1995; 11(6):62-68.
The effect of "medium-sized-peptides" of serum on chromatin structure and tamplet activity
1Protas A. F.
  1. Ukrainian Research Institute of Oncology and Radiology of Ministry of Health of Ukraine
    33/43, Lomonosova Str., Kyiv, Ukraine, 252022


The effect of serum "medium-sized-peptides" on RNA and DNA synthesis and chromatin binding in isolated cortical neurones was investigated. Peptides were purified from the serum of individuals exposed to radioactivity following the Chemobil accident. It was showed that peptides inhibited RNA and DNA synthesis and altered nucleosomal and supranucleosomal chromatin structure according to test with DNAse II. MSP bound chromatin DNA in cooperative manner, an average Kass is 36 g/ml and Hill's coefficient is 3,8. About four can bind with one nucleosome. Binding of peptides with free DNA is not cooperative, but with about the same Kass.


[1] Kishkun AA, Kudinova AS, Ofitova AD, Mishurina RB. The importance of middle molecules in assessing the level of endogenous intoxication (a review of the literature). Voen Med Zh. 1990;(2):41-4.
[2] Tupikova ZA. Middle-molecular uremic toxins (review of the literature). Vopr Med Khim. 1983;29(1):2-10.
[3] Nikolaochik VV, Moin VM, Kirkovski? VV, Mazur LI, Lobacheva GA, Bychko GN, Baratashvili GG. A method of determining middle molecules. Lab Delo. 1991;(10):13-8.
[4] Boman HG. Antibacterial peptides: key components needed in immunity. Cell. 1991;65(2):205-7.
[5] Romeo D, Skerlavaj B, Bolognesi M, Gennaro R. Structure and bactericidal activity of an antibiotic dodecapeptide purified from bovine neutrophils. J Biol Chem. 1988;263(20):9573-5.
[6] Malachi T, Zevin D, Gafter U, Chagnac A, Slor H, Levi J. DNA repair and recovery of RNA synthesis in uremic patients. Kidney Int. 1993;44(2):385-9.
[7] Kubat B, Moren GM, von der Decken A. DNA-dependent RNA polymerases from rat brain, and RNA synthesis in glial and neuronal cell-enriched fractions, after protein restriction. J Neurochem. 1978;31(5):1143-8.
[8] Protas AF. Effect of low doses of irradiation on synthesis of DNA, RNA and proteins in cerebral cortex cells. Ukr Biokhim Zh. 1995;67(5):105-9.
[9] Protas AF, Chaialo PP. The effect of low doses of external gamma irradiation on the chromatin structure and activity of the histone-specific proteinases of rat brain cells. Radiobiologiia. 1991;31(5):733-8.
[10] Chaialo PP, Protas AF. Structure and properties of the cell nucleus chromatin in the brain of rats exposed to gamma-radiation. Ukr Biokhim Zh. 1992;64(3):34-8.
[11] Marquet R, Colson P, Matton AM, Houssier C, Thiry M, Goessens G. Comparative study of the condensation of chicken erythrocyte and calf thymus chromatins by di- and multivalent cations. J Biomol Struct Dyn. 1988;5(4):839-57.
[12] Kiryanov GI, Smirnova TA, Polyakov VYu. Nucleomeric organization of chromatin. Eur J Biochem. 1982;124(2):331-8.
[13] Likhachev LP. Age peculiarities repair of DNA caused by carcinogenic agents. Usp Sovrem Biol. 1990. 110,2(5):180-191.
[14] Xu J, Manning FC, Patierno SR. Preferential formation and repair of chromium-induced DNA adducts and DNA--protein crosslinks in nuclear matrix DNA. Carcinogenesis. 1994;15(7):1443-50.