Biopolym. Cell. 1995; 11(5):51-55.
http://dx.doi.org/10.7124/bc.0003FB
The physiological significance of nuclear dna structural domain disintegration: evidence for non-random DNA domain cleavage
1Solov'yan V. T., 1Andreev I. O.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

We examined the pattern of ordered high molecular weight DNA cleavage In plant tissues showing diverse proliferative status or differentiation level, as well as that in human cultured lymphoblastoma cells during serum starvation. It was demonstrated that in quiescent or differentiated tissues there occur increased high molecular weight DNA cleavage, with the cleaved DNA domains being distinct from non-cleaved ones by the genome sequence composition. Lymphoblastoid cell culturing in serum-free medium was shown to be accompanied by progressive accumulation of high molecular weight DNA fragments which is rapidly decreased after serum addition. Transient increase in high molecular weight DNA fragmentation was found to accompany by temporal changes in C-myc sequence localization within cleaved DNA domains. The results obtained suggest that ordered disintegration of nuclear DNA into high molecular weight DNA fragments may present the specific genome reaction accompanying the physiological changes in the cells and may be presumably implicated to reprogramming of gene expression.
Full text: (PDF, in Ukrainian)

References

[1] Solov'ian VT, Andreev IO, Kunakh VA. Fractionation of eukaryotic DNA in a pulsed electrical field. II. Discrete DNA fragments and level of structural organization of chromatin. Mol Biol (Mosk). 1991;25(6):1483-91.
[2] Filipski J, Leblanc J, Youdale T, Sikorska M, Walker PR. Periodicity of DNA folding in higher order chromatin structures. EMBO J. 1990;9(4):1319-27.
[3] Razin SV, Petrov P, Hancock R. Precise localization of the alpha-globin gene cluster within one of the 20- to 300-kilobase DNA fragments released by cleavage of chicken chromosomal DNA at topoisomerase II sites in vivo: evidence that the fragments are DNA loops or domains. Proc Natl Acad Sci U S A. 1991;88(19):8515-9.
[4] Cohen GM, Sun XM, Fearnhead H, MacFarlane M, Brown DG, Snowden RT, Dinsdale D. Formation of large molecular weight fragments of DNA is a key committed step of apoptosis in thymocytes. J Immunol. 1994;153(2):507-16.
[5] Walker PR, Kokileva L, LeBlanc J, Sikorska M. Detection of the initial stages of DNA fragmentation in apoptosis. Biotechniques. 1993;15(6):1032-40.
[6] Oberhammer F, Wilson JW, Dive C, Morris ID, Hickman JA, Wakeling AE, Walker PR, Sikorska M. Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation. EMBO J. 1993;12(9):3679-84.
[7] Brown DG, Sun XM, Cohen GM. Dexamethasone-induced apoptosis involves cleavage of DNA to large fragments prior to internucleosomal fragmentation. J Biol Chem. 1993;268(5):3037-9.
[8] Collins SJ. The HL-60 promyelocytic leukemia cell line: proliferation, differentiation, and cellular oncogene expression. Blood. 1987;70(5):1233-44.
[9] Osheroff N. Biochemical basis for the interactions of type I and type II topoisomerases with DNA. Pharmacol Ther. 1989;41(1-2):223-41.
[10] Liu LF. DNA topoisomerase poisons as antitumor drugs. Annu Rev Biochem. 1989;58:351-75.
[11] Smith HC, Berezney R. Nuclear matrix-bound deoxyribonucleic acid synthesis: an in vitro system. Biochemistry. 1982;21(26):6751-61.
[12] Heller C, Pohl FM. A systematic study of field inversion gel electrophoresis. Nucleic Acids Res. 1989;17(15):5989-6003.
[13] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 344-50.
[14] Solovyan VT, Andreyev IO, Kunakh VA. The functional organization of plant nuclear DNA. I. Evidence for nuclear topoisomerase II. DNA complex. Mol Biol (Mosk). 1993; 27(6):1245-51.