Effect of native and modificated e-DNA on biosynthesis of photosynthetic pigments in Nicotiana tabacum L. 1. Content of chlorophylls and carotenoides in the plants of the first generation

Potopalsky, A. I.; Katsan, V. A.; Les'kiv, M. E.

doi:10.7124/bc.0003E5

Biopolym. Cell. 1995; 11(2):88-99.

http://dx.doi.org/10.7124/bc.0003E5

Effect of native and modificated e-DNA on biosynthesis of photosynthetic pigments in Nicotiana tabacum L. 1. Content of chlorophylls and carotenoides in the plants of the first generation

1Potopalsky A. I., 1Katsan V. A., 1Les'kiv M. E.

Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Effect of the native e-DNA from the salt-resistant solanum, n-plasmid and pCAMVNEO and also the native and alkylated DNA from the same origins have been studied on the biosynthesis of chlorophylls and carotenoides in the yellow-leaves tobacco cultivar Krupnolystny 20. e-DNA and e-DNT was acted on the seeds. It has been shown that general action of the investigated e-DNA and e-DNT is increasing of the level of the chlorophylls content and growth of the ratio between green and yellow pigments in the leaves of the beginning to blossom plants of the first generation.

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References

[1] Kartel NA. Effects of exogenous DNA in higher plants. Minsk: Nauka i tekhnika, 1981; 143 p.

[2] Kunakh VA, Potopal'skiy AI, Tkachuk ZYu, Alpatova LK.Normalization of abnormal karyotype in a population of haplopappus cultured cells under the influence modified RNA. Molekulyarnaya biologiya (Kiev). 1982; Is. 32: 52-6.

[3] Mashtaler SG, Yurkevich LP, Potopal'skiy AI. Cytogenetic effect of exogenous DNA on rye seeds of different ploidy. Genetika. 1988. 24(8):1419-28.

[4] Morgun VN, Tkachenko LV, Larchenko VA. Study of mutagenic activity of exogenous DNA in maize. Molekulyarnaya biologiya (Kiev). 1982; iss 32: 76-80.

[5] Morgun VN, Larchenko EA, Tkachenko LV, Potopalsky AI, Tkachuk ZYu. Comparative study of mutagenic activity of native and modified DNA in maize. Tsitol Genet. 1983; 17(4):58-61.

[6] Morgun VK, Larchenko VA. Mutagenic activity of exogenous DNA prospects of their applacation in the mutation breeding. Tsitol Genet. 1986; 20(11):46-50.

[7] Morgun VK, Logvinenko VF, Potopal'skiy AI, Bazhenova TM. Mutagenic activity of exogenous DNA into winter wheat. Dokl Akad Nauk Ukr SSSR Ser B. 1988; 8: 71-73.

[8] Potopal'skiy AI. Characteristics of tomatoes "Ukrainian salt tolerance" obtained by a modified DNA. Ed. recommendations to the way we use resources rastititelnyh Chernivtsi region. Chernivtsi, 1984: 13.

[9] Potopal'skiy AI, Yurkevich LP, Mashtaler SG. Using drugs exogenous nucleic acids in the selection of winter rye by shortness. Selektsiya i semenovodstvo. 1992;4(5):5-8.

[10] Glavinich RD, Toropkin MN, Zhidkova VA. Effect of exogenous DNA on heredity tomatoes.Selektsiya i semenovodstvo. 1985; 6:20-1.

[11] Mashtaler SG, Potopal'skiy AI, Yurkevich LP. The use of exogenous nucleic acids for breeding and valuable forms of cereals. Ecol. genetics of plants and animals, Proc. Conf. III All-Union. conf. Kishinev: Shtnintsa, 1987: 74.

[12] Baranov. EG Genetic features of inheritance of the chemical composition of tobacco varieties and their use in selection: Author. Dis .... Kand. biol. nauk. Echmiadzin, 1990, 25 p.

[13] Burlakina AV, Dyachkin II. Objective determination of the quality of raw tobacco. Sb. scientific. issled. Vitim works. Krasnodar, 1977, Vol. 166: 106-111.

[14] Piruzyan ES. Based on genetic engineering of plants. Nauka, 1988. 304 p.

[15] Fromm ME, Taylor LP, Walbot V. Stable transformation of maize after gene transfer by electroporation. Nature. 1986 Feb 27-Mar 5;319(6056):791-3.

[16] Patskovsky YuV, Gaiduk VV, Veselovsky OV, Zubko EI, Pasternak TP, Yurkevitch LN, Mashtaler SG, Potopalsky AI. Detection of pUC19-homologous repeated sequences into genome of several species of higher plants. Biopolym Cell. 1992; 8(3):23-9.

[17] Voloshchuk TP, Patskovskiĭ IuV, Potopal'skiĭ AI. Alkylation of nucleic acid components by ethyleneimine derivatives. I. Alkylation of bases. Bioorg Khim. 1990;16(7):981-90.

[18] Evstigneev VB, Prokhorova LI. On determination of chlorophylls a and b in mixture without separation of the components. Biokhimiia. 1968;33(2):286-95.

[19] Shlyk AA. On spectrophotometric determination of chlorophylls a and b. Biokhimiia. 1968;33(2):275-85.

[20] von Wettstein D. Chlorophyll lethals & submicroscopic morphological changes in plastids. Exp Cell Res. 1957;12(3):427-506.

[21] Plokhinskiy NA. Biometrics. Moscow, University Press, 1970. 367 p.

[22] Rokitsky PF. Biological Statistics. Minsk: Vysheyshaya wk., 1967. 328 p.

[23] Dunin MS, Popova NN. Drip method of diagnosis in plant viruses. M.: Selhozizdat, 1937; 47 p.