Relative content and transcriptional activity of liver chromatin fractions of rats of different ages

Mozzhukhina, T. G.; Litoshenko, A. Ya.

doi:10.7124/bc.0003E2

Biopolym. Cell. 1995; 11(2):69-75.

http://dx.doi.org/10.7124/bc.0003E2

Relative content and transcriptional activity of liver chromatin fractions of rats of different ages

1Mozzhukhina T. G., 1Litoshenko A. Ya.

Gerontology Institute of the Academy of Medical Sciences of Ukraine
67, Vyshgorodska Str., Kyiv, Ukraine, 04114

Abstract

The repressed (RC), transcriptionally active (AC), and matrix-bound (MC) chromatin fractions were isolated from liver cell nuclei of young, adult and old rats. It was shown that relative content of RC increased and that of AC decreased during aging. This decrease was accompanied by a rise of the protein/DNA ratio. Nevertheless, the intensive and extensive transcriptional activity indexes decreased with aging in all chromatin fractions. As a result the total RNA synthesis diminished, with a relative contribution to this synthesis of AC being decrease and of RC being increase.

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