Biopolym. Cell. 1995; 11(2):46-51.
Analysis of DNA from strains of malaria parasites sensitive and resistant to chloroquine by restriction endonuclease fingerprinting
1Pankova T. G., 1Igonina T. M., 1Klimova E. R., 1Mayer T. V.
  1. Institute of Cytology and Genetics, Siberian Branch of the RAS
    10, Akademika Lavrentieva Ave., Novosibirsk, Russian Federation, 630090

Abstract

DNAs from various Plasmodium berghei strains with different chloroquine (Chi) sensi­tivities were analysed by agarose gel electrophoresis following digestion with restriction endonucleases EcoRI, Hindlll and BamHI. The DNA patterns for the two Chl-resistant strains – LNK-65 strain with 2–3 fold increased Chl-resistance and LNK ChlR line with 7–10 fold increased, Chl-resistance (derived from the parent LNK-65 strain by stepwise selection in Chi) show similarity, but some additional bands of DNA digests are observed for LNK ChlR strain compared to the parent LNK strain. DNAs of ma­larial parasites from the sensitive N strain and from the Chl-resistant strains (LNK-65 and LNK ChlR) yield recognizably distinct patterns, so the resistant strains are sugges­ted to belong to another Plasmodium species.

References

[1] Bostock CJ, Tyler-Smith C. Genome DNA changes in methotrexate-resistant cells. Genome evolution. M. : Mir, 1986: 79-100.
[2] Rabinovich SA. Morphology of the strains of Plasmodium berghei berghei with "acquired" resistance to antimalarial drugs of different chemical groups. I. Morphologic features of strains resistant to derivatives of 4-aminoquinoline and diaminopyrimidine. Med Parazitol (Mosk). 1969;38(3):287-94.
[3] Rabinovich SA, Tikhomirova LA. Selective invasion of reticulocytes by parasites of Plasmodium berghei berghei strain with acquired resistance to chloroquine. Med Parazitol (Mosk). 1973;42(4):401-6.
[4] Dore E, Birago C, Frontali C, Battaglia PA. Kinetic complexity and repetitivity of Plasmodium berghei DNA. Mol Biochem Parasitol. 1980;1(4):199–208.
[5] Fulton JD, Grant PT. The sulphur requirements of the erythrocytic from of Plasmodium knowlesi. Biochem J. 1956;63(2):274-82.
[6] Gutteridge WE, Trigg PI, Williamson DH. Properties of DNA from some malarial parasites. Parasitology. 1971;62(2):209-19.
[7] Panyim S, Wilard P, Yuthavong J. Application of genetic engineering to research on tropical disease pathogenes with special reference to Plasmodia. Geneva : WHO, 1986.
[8] Peters W, Chance ML, Lissner R, Momen H, Warhurst DC. The chemotherapy of rodent malaria, XXX. The enigmas of the 'NS lines' of P. berghei. Ann Trop Med Parasitol. 1978;72(1):23-36.
[9] Salganik RI, Pankova TG, Chekhonadskikh TV, Igonina TM. Chloroquine resistance of Plasmodium berghei: biochemical basis and countermeasures. Bull World Health Organ. 1987;65(3):381-6.
[10] Alt FW, Kellems RE, Bertino JR, Schimke RT. Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells. J Biol Chem. 1978;253(5):1357-70.
[11] Wilson CM, Serrano AE, Wasley A, Bogenschutz MP, Shankar AH, Wirth DF. Amplification of a gene related to mammalian mdr genes in drug-resistant Plasmodium falciparum. Science. 1989;244(4909):1184-6.
[12] Wilson RJ, Farrant J, Walter CA. Preservation of intraerythrocytic forms of malarial parasites by one-step and two-step cooling procedures. Bull World Health Organ. 1977;55(2-3):309-15.