The way of receiving and cultivating of human embryonic hepatocytes

Sukhorada, H. M.; Lukash, L. L.; Podolskaya, S. V.; Shved, A. D.; Smykodub, A. I.

doi:10.7124/bc.0003D9

Biopolym. Cell. 1995; 11(1):92-94.

http://dx.doi.org/10.7124/bc.0003D9

The way of receiving and cultivating of human embryonic hepatocytes

1Sukhorada H. M., 1Lukash L. L., 1Podolskaya S. V., 1Shved A. D., 1Smykodub A. I.

Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

A simple method of giving of human embryonic hepatocytes have been described. It allows to receive up to 90 % cells of great vitality. Liver was treated by fermental-mechanic way and cultivated at (the temperature of 37 °C in the 5 % CO₂ atmosphere during 12–14 days.

Full text: (PDF, in Russian)

References

[1] Freshney R. Culture of Animal Cells: A Manual of Basic Technique. Wiley-Blackwell 1987.

[2] Bliumkin VN, Skaletskiĭ NN, Babikova RA, Kirsanova LA, Zagrebina OV. Preparation of liver cultures from human embryos. Biull Eksp Biol Med. 1988;105(2):212-4.

[3] Gichev YU. P., Graudinya ZH. P. Culture of liver tissue in hepatology. Novosibirsk: Nauka, 1986. 88.p

[4] Grishchenko V. I., Lobyntseva G. S, Votyakova I. A., Shereshkov S. I. Hematopoietic cells of fetal liver. Embryogenesis, cryopreservation and transplantation. Kiev: Naukova Dumka, 1988. 192 p.

[5] A. p. 1601222. Method for producing hepatocytes. N. A. Onishchenko, M. A. Danilov S. D. Artamonov. publ 08.08.1990

[6] Eraĭzer TL, Grinberg KN, Vorsanova SG. Characteristics of human embryonal hepatocytes cultivated in vitro. Biull Eksp Biol Med. 1975;79(5):123-5. Russian.