Biopolym. Cell. 1995; 11(1):81-86.
Investigation of autoantibodies directed against tissue-specific myocardial antigens in dilated cardiomyopathy
1Sidorik L. L., 1Rodnin N. V., 1Bobyk V. I., 1Ryabenko D. V., 1Veberov A. V., 1Tkachenko T. Yu., 1Matsuka G. Kh.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The autoantibodies directed against actin and tnyosine have been detected in the blood sera of patients with dilated cardiomyopathy (DCMP) at the level that was sufficiently higher than in healthy donors. While comparing of the immunogeneicity of these proteins we have stated that myosine from affected by DCMP myocardium have been more immunogenic then from normal one. In the case of actin the immunogeneicity have been higher for the protein from normal myocard. The investigations of actin by limited proteolysis may suggest that the differencies in immune properties are not connected with primary structure but with structures of higher levels.

References

[1] Rose NR, Neumann DA, Herskowitz A. Autoimmune myocarditis: concepts and questions. Immunol Today. 1991;12(8):253-5.
[2] Schultheiss HP, Bolte HD. Immunological analysis of auto-antibodies against the adenine nucleotide translocator in dilated cardiomyopathy. J Mol Cell Cardiol. 1985;17(6):603-17.
[3] Neumann DA, Burek CL, Baughman KL, Rose NR, Herskowitz A. Circulating heart-reactive antibodies in patients with myocarditis or cardiomyopathy. J Am Coll Cardiol. 1990;16(6):839-46.
[4] Limas CJ, Limas C. Immune-mediated modulation of beta-adrenoceptor function in human dilated cardiomyopathy. Clin Immunol Immunopathol. 1993;68(2):204-7.
[5] Limas CJ, Limas C, Kubo SH, Olivari MT. Anti-beta-receptor antibodies in human dilated cardiomyopathy and correlation with HLA-DR antigens. Am J Cardiol. 1990;65(7):483-7.
[6] Limas CJ, Goldenberg IF, Limas C. Assessment of immune modulation of beta-adrenergic pathways in human dilated cardiomyopathy: influence of methodologic factors. Am Heart J. 1992;123(4 Pt 1):967-70.
[7] Limas CJ, Limas C. HLA-DRw6 antigen linkage in chronic congestive heart failure secondary to coronary artery disease (ischemic cardiomyopathy). Am J Cardiol. 1988;62(10 Pt 1):816-8.
[8] Limas C, Limas CJ, Boudoulas H, Graber H, Bair R, Sparks L, Wooley CF. T-cell receptor gene polymorphisms in familial cardiomyopathy: correlation with anti-beta-receptor autoantibodies. Am Heart J. 1992;124(5):1258-63.
[9] Rose NR, Herskowitz A, Neumann DA, Neu N. Autoimmune myocarditis: a paradigm of post-infection autoimmune disease. Immunol Today. 1988;9(4):117-20.
[10] Bobyk V. I., Veberov A. V., Ryabenko D. V., Dubrovskaya G. V., Rodnin N. V., Sidorik L. L. The purification of the main tissue-specific antigens from the normal and effected by dilatative cardiomyopathia human heart. Biopolym. Cell. 1993; 9(6):63-66.
[11] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.
[12] Goosen PC. Isolation of subclasses of human IgG with affinity chromatography. J Immunol Methods. 1980;37(1):89-93.
[13] Matsiota P, Druet P, Dosquet P, Guilbert B, Avrameas S. Natural autoantibodies in systemic lupus erythematosus. Clin Exp Immunol. 1987;69(1):79-88.
[14] Ternynck T, Bleux C, Gregoire J, Avrameas S, Kanellopoulos-Langevin C. Comparison between autoantibodies arising during Trypanosoma cruzi infection in mice and natural autoantibodies. J Immunol. 1990;144(4):1504-11.
[15] Kozlov EA, Rodnin NV, Levitina TL, Gusak NM, Radomskij NF, Palchikovskaya LJ. The amino acid sequence determination of a granulin and polyhedrin from two baculoviruses infecting Agrotis segetum. Virology. 1992;189(1):320-3.
[16] Gething MJ, Sambrook J. Protein folding in the cell. Nature. 1992;355(6355):33-45.
[17] Alexander H, Alexander S, Getzoff ED, Tainer JA, Geysen HM, Lerner RA. Altering the antigenicity of proteins. Proc Natl Acad Sci U S A. 1992;89(8):3352-6.
[18] Epstein HF, Fischman DA. Molecular analysis of protein assembly in muscle development. Science. 1991;251(4997):1039-44.
[19] Lange LG, Schreiner GF. Immune mechanisms of cardiac disease. N Engl J Med. 1994;330(16):1129-35.
[20] Naparstek Y, Plotz PH. The role of autoantibodies in autoimmune disease. Annu Rev Immunol. 1993;11:79-104.
[21] Plotz PH. Autoantibodies are anti-idiotype antibodies to antiviral antibodies. Lancet. 1983;2(8354):824-6.
[22] Schwartz RS. Autoantibodies and normal antibodies. Progr Immunol. 1986; 6: 478-482.