Biopolym. Cell. 1995; 11(1):61-65.
http://dx.doi.org/10.7124/bc.0003D4
Lysogeny by MS2 phage. Analysis of a recombinant plasmid containing MS2 RNA-like sequence
1Pererva T. P., 1Mirjuta N. Yu., 1Mirjuta A. Yu., 1Woodmaska M. I., 1Zherebtsova E. N.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

pL34 recombinant plasmid coding MS2-llke RNA synthesis in plasmid-containing cells has been studied using physical mapping and blot-hybridisation with P32-labelled pMS27 plasmid fragments including a DNA copy of MS2 phage RNA. The authors have determined the size of a fragment containing MS2-like sequence and its localisation inside the hybrid plasmid.
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References

[1] Pererva TP. Resistance to phage MS2 induced in E. coli by infection with that phage. Tsitol Genet. 1977;11(1):3-9.
[2] Pererva TP Malyuta SS. E. coli System L152-induced mutants on the F-factor. Molecular biology. Molekulyarnaya biologiya (Kiev). 1984. 38: 81-90.
[3] Pererva T. P., Miryuta N. Yu., Miryuta A. Yu. MS2 phage lysogeny. Phage-specific RNA synthesis on cell DNA. Biopolym. Cell. 1993; 9(1):45-50.
[4] Kopylova-Sviridova TN, Fodor I. New vector plasmids for selecting recombinant clones of E. coli. Dokl Akad Nauk SSSR. 1979;245(5):1250-3.
[5] Devos R, van Emmelo J, Contreras R, Fiers W. Construction and characterization of a plasmid containing a nearly full-size DNA copy of bacteriophage MS2 RNA. J Mol Biol. 1979;128(4):595-619.
[6] Devos R, Contreras R, van Emmelo J, Fiers W. Identification of the translocatable element IS1 in a molecular chimera constructed with plasmid pBR322 DNA into which a bacteriophage MS2 DNA copy was inserted by the poly(dA).poly(dT) linker method. J Mol Biol. 1979;128(4):621-32.
[7] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning : a laboratory manual Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory, 1982. 545p
[8] Fitch WM, Smith TF, Ralph WW. Mapping the order of DNA restriction fragments. Gene. 1983;22(1):19-29.
[9] Fiers W, Contreras R, Duerinck F, Haegeman G, Iserentant D, Merregaert J, Min Jou W, Molemans F, Raeymaekers A, Van den Berghe A, Volckaert G, Ysebaert M. Complete nucleotide sequence of bacteriophage MS2 RNA: primary and secondary structure of the replicase gene. Nature. 1976;260(5551):500-7.
[10] Berkhout B, van Duin J. Mechanism of translational coupling between coat protein and replicase genes of RNA bacteriophage MS2. Nucleic Acids Res. 1985;13(19):6955-67.