The influence of the implanted cloning human\'s ApoAI gene on the development of rabbit\' hypercholesterinemy

Frolkis, V. V.; Kordyum, V. A.; Novikova, S. N.; Bogatskaya, L. N.; Irodov, D. M.; Likhacheva, L. I.; Potapenko, R. I.; Mozguhina, T. G.; Bitner, M. K.

doi:10.7124/bc.0003CB

Biopolym. Cell. 1994; 10(6):98-105.

http://dx.doi.org/10.7124/bc.0003CB

The influence of the implanted cloning human's ApoAI gene on the development of rabbit' hypercholesterinemy

1Frolkis V. V., 2Kordyum V. A., 2Novikova S. N., 1Bogatskaya L. N., 2Irodov D. M., 2Likhacheva L. I., 2Potapenko R. I., 1Mozguhina T. G., 1Bitner M. K.

Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Gerontology Institute of the Academy of Medical Sciences of Ukraine
67, Vyshgorodska Str., Kyiv, Ukraine, 04114

Abstract

The effect of the implanted cloning human's apoAl gene on the dynamic of rabbit's lipid and lipoproteid metabolism in conditions of development of experimental hypercholesterinemy was investigated. The implantation of apoAl gene results in the appearance of human apolipoprotein Al in the blood of experimental animals, the changes in the content of cholesterine and it's shifts in different fractions of LP, the changes in the ratio of different classes of LP in favour Chs-HDLP and HDLP. It is supposed that transfection of apoAl human's gene and it's transitory expression leads not only to the biosynthesis of respective protein but also to a change in the work of total genome via gene-regulatory mechanisms.

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