Biopolym. Cell. 1994; 10(6):88-91.
http://dx.doi.org/10.7124/bc.0003C9
Individual isoacceptor tyrosine trnas from bovine liver. isolation and characteristic
1Kalachnyuk L. G.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Combination of two chromatographies of BD-cellulose in the presence and in the absence of Mg2+ was applied for high yield isolation of individual isoacceptor tyrosine tRNAs from bovine liver. The final purifacation of tyrosine tRNA was achieved by gel electrophoresis on a 7 % gel at 7 M urea. The nucleotide sequence of tRNATyr1 from bovine liver was determined to be identic to primaty structure of tyrosine tRNA Q·ψA from bovine liver (13) ( minor bases was not determined).
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References

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