Biopolym. Cell. 1994; 10(5):98-104.
http://dx.doi.org/10.7124/bc.0003BF
The DNA probe construction with use the silver salt. 2. The colouring of the monomer and the single-stranded DNA
1Lichina M. V., 1Shugalii A. V.
  1. Institute of Chemical Physics, RAS in Chernogolovka
    Academician Semenov avenue 1, Chernogolovka, Moscow region, Russian Federation, 142432

Abstract

Some aspects of adenine modification as a one of DNA base during the preparing of coloured metal-complex is under investigation. After the N-metoxyadenine formation as a result of formaldehyde reaction with the amino/imino groups of the base the lengthing of lateral chain is occured at the expense of ethanolamine and some metoxy groups. The colour of modified adenine is developed after a silvre addition and the ultimate branched metal-complex possess in addition the fluorescent properties. The kind of clour is determined by pH of incubation media, the violet probe with a 10–15 % modification level is obtained in an alkaline solution in the course of 15–20 minutes.
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References

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