Biopolym. Cell. 1994; 10(5):68-77.
http://dx.doi.org/10.7124/bc.0003BC
Levels of rna for TNF-α and receptors during the prereplicative period of liver regeneration
1Obolenskaya M. Yu., 2Bernauer H., 2Tran-Thi T.-A., 2Decker K.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute for Biochemistry and Molecular Biology, Albert-Ludwigs-University of Freiburg
    Stefan-Meier-Strase 17, D-79104 Freiburg, Germany

Abstract

During the prereplicative period of liver regeneration the changes of the tnRNA contents for tumor necrosis factor-α (TNF-α) and its receptors were examined in total liver RNA with the help of reverse transcriptase-polymera.se chain reaction (RT-PCR) and compared with those after sham operation or stimulation with lipopolysaccharide (LPS). In regenerating liver all the changes are nearly synchronous with a slight delay (or the TNF receptor RNAs. The mRNA levels reach their maximum at 1–3 h after operation and exceed the values for intact animals about ten-fold. The corresponding changes induced by sham operation are quantitatively less than those in the regenerating liver and manifest themselves at the end of the prereplicative period. LPS stimulation induced an increase of TNF-α and TNF receptor production comparable with during regeneration. Analysis of the expression of the 55 kDa TNF receptor revealed qualitative changes, e. g. an increased polyadenylation and an imbalance between the amplification of the whole molecule and its 5'-terminal half pointing to as yet unidentified changes at the 3' end of the molecule.
Full text: (PDF, in English)

References

[1] Fausto N, Mead JE. Regulation of liver growth: protooncogenes and transforming growth factors. Lab Invest. 1989;60(1):4-13.
[2] Denhardt DT, Edwards DR, Parfett CL. Gene expression during the mammalian cell cycle. Biochim Biophys Acta. 1986;865(2):83-125.
[3] Iakovlev AIu. Hepatocyte dynamic reserve: the mechanism for the assurance of specialized functions in the regenerating liver. Tsitologiia. 1979;21(11):1243-52.
[4] Epifanova OI, Terskih VV, Polunovskij VA. Quiescent cells. Characteristics and functions in organism. M.: Nauka, 1983. 176 p.
[5] Obolenskaia MIu, Gerasimova TB, Bilich KM, Platonov OM. Intracellular distribution of newly formed hepatocyte RNA in the 1st hours following partial hepatectomy. Tsitol Genet. 1987;21(5):376-82.
[6] Decker K. Biologically active products of stimulated liver macrophages (Kupffer cells). Eur J Biochem. 1990;192(2):245-61.
[7] Decker K. Basic Mechanisms of the Inflammatory Response. In: Molecular aspects of inflammation. Eds. H Sies, L Flohe, G Zimmer. Berlin, Heidelberg : Springer, 1991: 1-23.
[8] Camussi G, Albano E, Tetta C, Bussolino F. The molecular action of tumor necrosis factor-alpha. Eur J Biochem. 1991;202(1):3-14.
[9] Czaja MJ, Flanders KC, Biempica L, Klein C, Zern MA, Weiner FR. Expression of tumor necrosis factor-alpha and transforming growth factor-beta 1 in acute liver injury. Growth Factors. 1989;1(3):219-26.
[10] Cornell RP, Liljequist BL, Bartizal KF. Depressed liver regeneration after partial hepatectomy of germ-free, athymic and lipopolysaccharide-resistant mice. Hepatology. 1990;11(6):916-22.
[11] Akerman P, Cote P, Yang SQ, McClain C, Nelson S, Bagby GJ, Diehl AM. Antibodies to tumor necrosis factor-alpha inhibit liver regeneration after partial hepatectomy. Am J Physiol. 1992;263(4 Pt 1):G579-85.
[12] Mayanski DN, Scherbakoff VI. Biul Eksp Biol Med. 1979; 86: 69-71.
[13] Fisher B, Gebhardt MC, Saffer EA, Fisher ER. Effect of Corynebacterium parvum on liver proliferation and regeneration. Cancer Res. 1979;39(4):1361-8.
[14] Kronke M, Schutze S, Scheurich P, Pfizenmaeir K. Tumor Necrosis Factor. Eds BB Aggarwal, J Vilcek. Dekker, etc., 1992: 189-216.
[15] Xanthopoulos KG, Mirkovitch J, Decker T, Kuo CF, Darnell JE Jr. Cell-specific transcriptional control of the mouse DNA-binding protein mC/EBP. Proc Natl Acad Sci U S A. 1989;86(11):4117-21.
[16] Mischoulon D, Rana B, Bucher NL, Farmer SR. Growth-dependent inhibition of CCAAT enhancer-binding protein (C/EBP alpha) gene expression during hepatocyte proliferation in the regenerating liver and in culture. Mol Cell Biol. 1992;12(6):2553-60.
[17] Higgins GM, Anderson RM. Experimental pathology of the liver. Arch Pathol. 1931; 12(2):186-202.
[18] Eyhorn S, Schlayer HJ, Henninger HP, Dieter P, Hermann R, Woort-Menker M, Becker H, Schaefer HE, Decker K. Rat hepatic sinusoidal endothelial cells in monolayer culture. Biochemical and ultrastructural characteristics. J Hepatol. 1988;6(1):23-35.
[19] Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162(1):156-9.
[20] Sambrook J, Fritsch EE, Maniatis T. Molecular Cloning. New York : Cold Spring Harbor Lab., 1989. 625 p.
[21] Rychlik W, Rhoads RE. A computer program for choosing optimal oligonucleotides for filter hybridization, sequencing and in vitro amplification of DNA. Nucleic Acids Res. 1989;17(21):8543-51.
[22] Flick DA, Gifford GE. Comparison of in vitro cell cytotoxic assays for tumor necrosis factor. J Immunol Methods. 1984;68(1-2):167-75.
[23] Estler HC, Grewe M, Gaussling R, Pavlovic M, Decker K. Rat tumor necrosis factor-alpha. Transcription in rat Kupffer cells and in vitro posttranslational processing based on a PCR-derived cDNA. Biol Chem Hoppe Seyler. 1992;373(5):271-81.
[24] Gurevich VV, Pokrovskaya ID, Obukhova TA, Zozulya SA. Preparative in vitro mRNA synthesis using SP6 and T7 RNA polymerases. Anal Biochem. 1991;195(2):207-13.
[25] Fuchs P, Strehl S, Dworzak M, Himmler A, Ambros PF. Structure of the human TNF receptor 1 (p60) gene (TNFR1) and localization to chromosome 12p13 [corrected]. Genomics. 1992;13(1):219-24.
[26] Himmler A, Maurer-Fogy I, Krönke M, Scheurich P, Pfizenmaier K, Lantz M, Olsson I, Hauptmann R, Stratowa C, Adolf GR. Molecular cloning and expression of human and rat tumor necrosis factor receptor chain (p60) and its soluble derivative, tumor necrosis factor-binding protein. DNA Cell Biol. 1990;9(10):705-15.
[27] Mochida S, Ohta Y, Ogata I, Fujiwara K. Gut-derived substances in activation of hepatic macrophages after partial hepatectomy in rats. J Hepatol. 1992;16(3):266-72.
[28] Blouin A, Bolender RP, Weibel ER. Distribution of organelles and membranes between hepatocytes and nonhepatocytes in the rat liver parenchyma. A stereological study. J Cell Biol. 1977;72(2):441-55.
[29] Geerts A, Greenwel P, Cunningham M. et al. Cells of the hepatic sinusoid. Eds E. Wisse, D. L. Knook, R. S. McCuskey. Leiden : Kupffer Cell foundation, 1991; Vol. 3:150-4.
[30] Tartaglia LA, Weber RF, Figari IS, Reynolds C, Palladino MA Jr, Goeddel DV. The two different receptors for tumor necrosis factor mediate distinct cellular responses. Proc Natl Acad Sci U S A. 1991;88(20):9292-6.
[31] Mayanski DN, Scherbakoff VI, Grishanova AU. Multiple esterase forms in isolated hepatocytes and Kupffer cells of partially hepatectomized rats. Experientia. 1980;36(7):856-7.
[32] Obolenskaya MYu, Prima VI, Gerasimova TB, Platonov OM. Partial restriction of genome expression – a component of its work reprogramming in regenerating liver of mammals. Biopolym. Cell. 1989;5(2):79-88.
[33] Lengyel P. Biochemistry of interferons and their actions. Annu Rev Biochem. 1982;51:251-82.
[34] Pestka S, Langer JA, Zoon KC, Samuel CE. Interferons and their actions. Annu Rev Biochem. 1987;56:727-77.