Biopolym. Cell. 1994; 10(3-4):103-104.
Indirect determination of the 5'→3' and 3'→5' of the DNA-dependent DNA-polymerase function
1Skripal' I. G., 1Bezugly S. V., 1Babitschev V. V.
  1. D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
    154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

Doxorubicin in the concentration of 1 mg/ml and streptonigrin in the concentration 1000 mg/ml each agent separately should by useful to determination of replicative (5'→3') or reparatlve (3'→5') function DNA-polymerase. This enzymes with 5'→3' fully inhibited by doxorubicine but net sensitive to action of streptonigrin in concentration substances mentioned above. And contraverse, DNA-polymerase with 3'→5' function don't sensitive to doxorubicin and it's activity depressed by streptonigrin.

References

[1] Chuang RY, Chuang LF. Inhibition of chicken myeloblastosis RNA polymerase II activity by adriamycin. Biochemistry. 1979;18(10):2069-73.
[2] Galf EF, Cundliff E, Reynolds PE. et at. The molecular basis of antibiotic action. Ed. A Wiley London; New York: John Wiley and Song, 1972. 500 p.
[3] Bauer GA, Heller HM, Burgers PM. DNA polymerase III from Saccharomyces cerevisiae. I. Purification and characterization. J Biol Chem. 1988;263(2):917-24.
[4] Kedinger C, Gniazdowski M, Mandel JL Jr, Gissinger F, Chambon P. Alpha-amanitin: a specific inhibitor of one of two DNA-pendent RNA polymerase activities from calf thymus. Biochem Biophys Res Commun. 1970;38(1):165-71.
[5] Roeder RG. Multiple forms of deoxyribonucleic acid-dependent ribonucleic acid polymerase in Xenopus laevis. Isolation and partial characterization. J Biol Chem. 1974;249(1):241-8.
[6] Byrnes JJ, Downey KM, Black VL, So AG. A new mammalian DNA polymerase with 3' to 5' exonuclease activity: DNA polymerase delta. Biochemistry. 1976;15(13):2817-23.
[7] Rüttimann C, Cotorás M, Zaldívar J, Vicuña R. DNA polymerases from the extremely thermophilic bacterium Thermus thermophilus HB-8. Eur J Biochem. 1985;149(1):41-6.