Indirect determination of the 5\'→3\' and 3\'→5\' of the DNA-dependent DNA-polymerase function

Skripal', I. G.; Bezugly, S. V.; Babitschev, V. V.

doi:10.7124/bc.0003B7

Biopolym. Cell. 1994; 10(3-4):103-104.

http://dx.doi.org/10.7124/bc.0003B7

Indirect determination of the 5'→3' and 3'→5' of the DNA-dependent DNA-polymerase function

1Skripal' I. G., 1Bezugly S. V., 1Babitschev V. V.

D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

Doxorubicin in the concentration of 1 mg/ml and streptonigrin in the concentration 1000 mg/ml each agent separately should by useful to determination of replicative (5'→3') or reparatlve (3'→5') function DNA-polymerase. This enzymes with 5'→3' fully inhibited by doxorubicine but net sensitive to action of streptonigrin in concentration substances mentioned above. And contraverse, DNA-polymerase with 3'→5' function don't sensitive to doxorubicin and it's activity depressed by streptonigrin.

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