Creation of the expressing constructions with bar gene for plant transformation

Zayas, A. I.; Stekhin, I. N.; Putilina, N. V.; Levenko, B. A.

doi:10.7124/bc.0003B4

Biopolym. Cell. 1994; 10(3-4):89-92.

http://dx.doi.org/10.7124/bc.0003B4

Creation of the expressing constructions with bar gene for plant transformation

1Zayas A. I., 1Stekhin I. N., 1Putilina N. V., 1Levenko B. A.

Institute of Plant Physiology and Genetics, NAS of Ukraine
31/17, Vasylkivska, Kyiv, Ukraine, 03022

Abstract

Phosphinothrioin (PPT) is a potent inhibitor of glutamine synthetase in bacteria and plants and is used as a non-selective herbicide. The bar gene which confers resistance ire Streptomyces hygroscopicus to bialaphos, a tripeptide containing PPT, was cloned. The bar gene was placed under control of the 35S promoter of the cauliflower mosaic virus for transferring to plant cells using agrobacterium–mediated transformation.

Full text: (PDF, in Russian)

References

[1] Botterman J, Leemans J. Engineering herbicide resistance in plants. Trends Genet. 1988;4(8):219-22.

[2] Greef WD, Delon R, Block MD, Leemans J, Botterman J. Evaluation of Herbicide Resistance in Transgenic Crops Under Field Conditions. Bio/Technology. 1989;7(1):61-4.

[3] Piruzian ES, Mett VL, Kobets NS, Urmeeva FI. The use of bacterial genes encoding herbicide tolerance in constructing transgenic plants. Microbiol Sci. 1988;5(8):242-8.

[4] Murakami T, Anzai H, Imai S, Satoh A, Nagaoka K, Thompson CJ. The bialaphos biosynthetic genes ofStreptomyces hygroscopicus: Molecular cloning and characterization of the gene cluster. Mol Gen Genet. 1986;205(1):42-53.

[5] Block MD, Botterman J, Vandewiele M, Dockx J, Thoen C, Gosselé V, Movva NR, Thompson C, Montagu MV, Leemans J. Engineering herbicide resistance in plants by expression of a detoxifying enzyme. EMBO J. 1987;6(9):2513-8.

[6] Yanisch-Perron C, Vieira J, Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103-19.

[7] Fromm ME, Taylor LP, Walbot V. Stable transformation of maize after gene transfer by electroporation. Nature. 1986 Feb 27-Mar 5;319(6056):791-3.

[8] Draper J, Scott R, Armitage P, Walden R, eds. Plant genetic transformation and gene expression: a Laboratory Manual. Oxford: Blackwell Scientific, 1988; 8-62.

[9] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.

[10] Koekman BP, Hooykaas PJ, Schilperoort RA. Localization of the replication control region on the physical map of the octopine Ti plasmid. Plasmid. 1980;4(2):184-95.

[11] Zayas AI, Stekhin IN, Putilina NV, Levenko BA. Cloning of the herbicide bialaphos resistance gene. Biopolym Cell. 1994; 10(3-4):84-8.

[12] Kuz'min YeV, Shadenkov AA, Uzbekova SV, Shemyakin MF. Construction of bifunctional gene derivatives of insectotoxin thuringiensis var. kurstaki for their expression in transgenic plants. Dokl Akad Nauk SSSR. 1991; 319(5):1248-51.

[13] Ditta G, Stanfield S, Corbin D, Helinski DR. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A. 1980;77(12):7347-51.