Biopolym. Cell. 1994; 10(3-4):84-88.
http://dx.doi.org/10.7124/bc.0003B3
Cloning of the herbicide bialaphos resistance gene
1Zayas A. I., 1Stekhin I. N., 1Putilina N. V., 1Levenko B. A.
  1. Institute of Plant Physiology and Genetics, NAS of Ukraine
    31/17, Vasylkivska, Kyiv, Ukraine, 03022

Abstract

A gene which confers resistance to the herbicide bialaphos (bar) has been cloned from Streptomyces hygroscopicus genomic DNA. The bar gene was cloned in E. coli using the vector pUCfff. The recombinant clones, resistant to bialaphos in concentration 0,005 mg/ml, were obtained. Plasmid DNA isolated from resistant clones in each case contained the same 1,7-kb PstI fragment. Restriction mapping of this fragment has been made.
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References

[1] Botterman J, Leemans J. Engineering herbicide resistance in plants. Trends Genet. 1988;4(8):219-22.
[2] Greef WD, Delon R, Block MD, Leemans J, Botterman J. Evaluation of Herbicide Resistance in Transgenic Crops Under Field Conditions. Bio/Technology. 1989;7(1):61-4.
[3] Block MD, Botterman J, Vandewiele M, Dockx J, Thoen C, GosselГ© V, Movva NR, Thompson C, Montagu MV, Leemans J. Engineering herbicide resistance in plants by expression of a detoxifying enzyme. EMBO J. 1987;6(9):2513-8.
[4] Murakami T, Anzai H, Imai S, Satoh A, Nagaoka K, Thompson CJ. The bialaphos biosynthetic genes ofStreptomyces hygroscopicus: Molecular cloning and characterization of the gene cluster. Mol Gen Genet. 1986;205(1):42-53.
[5] Piruzian ES, Mett VL, Kobets NS, Urmeeva FI. The use of bacterial genes encoding herbicide tolerance in constructing transgenic plants. Microbiol Sci. 1988;5(8):242-8.
[6] DNA cloning: a practical approach. Ed. DM Glover. Oxford, IRL Press, 1985; Vol. 1 and 2: 435 p
[7] Yanisch-Perron C, Vieira J, Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103-19.
[8] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.
[9] Draper J, Scott R, Armitage P, Walden R, eds. Plant genetic transformation and gene expression: a Laboratory Manual. Oxford: Blackwell Scientific, 1988; 28-15.
[10] Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975;98(3):503-17.
[11] Rigby PW, Dieckmann M, Rhodes C, Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977;113(1):237-51.
[12] Thompson CJ, Movva NR, Tizard R, Crameri R, Davies JE, Lauwereys M, Botterman J. Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus. EMBO J. 1987;6(9):2519-23.