Biopolym. Cell. 1994; 10(2):32-37.
Some peculiarities of para-fluorophenylalanine resistant macrotomia dyeing cell culture
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Abstract
Suspension culture of macrotomia dyeing (Arnebia/Macrotomia euchroma (Royle) Pauls) resistant to para-fluorophenylalanine (PFP) is characterized by low level of shikonin accumulation as compared to initial cell strain and retain this feature for a long time of growth in the absence of PFP. At the same time increase of resistance level is observed. Indolebutiric acid instead of indoleacetic acid and kinetin promote partial recovery of cell productivity. No rootformation usually observed in initial cell strain after such phytohormone exchange in medium is observed in PFP-resistant variant.
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References
[1]
Lhoas P. Mitotic haploidization by treatment of Aspergillus niger diploids with para-fluorophenylalanine. Nature. 1961;190:744.
[2]
Banks P, Britten EJ, Gordon GH. Para -Fluorophenylalanine-induced chromosome number changes in higher plants. I. Maize . Genome. 1989;32(6):962–6.
[3]
Niizeki M. Studies on plant cell and tissue culture. IV. Effect of para-fluorophenylalanine on haploid and diploid cells of tobacco plant in vitro. J Faculty Agric Hokkaido Univ. 1974; 57(4):349-56.
[4]
Fukui K, Niizeki H. Artificial reduction of chromosome number in Fragaria nanasa following treatment with p-fluorophenylalanine. Plant tissue culture: Proc. 5th Int. congr. plant tissue and cell culture (Tokyo, Lake Jamanaka, 1982). Tokyo, 1982:427-8.
[5]
Sisken JE. The effects of p-DL-fluorophenylalanine on chromosome movement and cytokinesis of human amnion cells in culture. Chromosoma. 1973;44(1):91-8.
[6]
Levenko BA, Kiforak OV. Effect of fluorophenylalanine on tissue culture and tomato gaplopappusa. Exp. plant genetics. Kiev: Naukova Dumka, 1977:138-142.
[7]
Noodén LD, Thimann KV. Action of inhibitors of RNA and protein synthesis on cell enlargement. Plant Physiol. 1966;41(1):157-64.
[8]
Orkwiszewski JAJ, Hopkins WG. Effect of Fluorophenylalanine on L-Phenylalanine Ammonia-Lyase Activity in Avena Coleoptiles. Am J Bot. 1974;61(4):433-6.
[9]
Zakhlenjuk OV, Kostenyuk IA. Some cyto- and morphogenetic effects of para-fluorophenylalanine in tissue cultures of Nicotiana tabacum and Papaver bracteatum. Biopolym Cell. 1991; 7(5):66-71.
[10]
Berlin J, Widholm JM. Correlation between Phenylalanine Ammonia Lyase Activity and Phenolic Biosynthesis in p-Fluorophenylalanine-sensitive and -resistant Tobacco and Carrot Tissue Cultures. Plant Physiol. 1977;59(4):550-3.
[11]
Salgado-Garciglia R, Ochoa-Alejo N. Increased capsaicin content in PFP-resistant cells of chili pepper (Capsicum annuum L.). Plant Cell Rep. 1990;8(10):617-20.
[12]
Quesnel AA, Ellis BE. Comparison of UV irradiation and p-fluorophenylalanine as selective agents for production of aromatic compounds in plant cell cultures. J Biotechnol.1989;10(1):27–37.
[13]
Zakhlenjuk OV, Vidmachenko TV, Kunakh VA. et al. Comparative characteristics of shikonin accumulating Arnebia euchroma suspension culture and it's p-fluo- rophenylalanine-resistant variant. Acta Horticulture. 1993; 330:293-8.
[14]
Davydenkov VP, Patudin AV, Popov KG, Rabinovich SA, Miroshnikoye AI. Cell Culture Arnebia euchroma (Royle) Jonst - new source of shikonin. Khim farm zhurn. 1991;1: 53-5.
[15]
Tabata M, Fujita Y. Production of shikonin by plant cell cultures. Biotechnol. in plant sci. Relevance to agriculture in eighties. Ed. M. Zaitlin. New York : Acad. press, 1985: 207-18.
[16]
Quesnel A, Ellis BE. Population responses in tobacco cell cultures during selection for resistance to para-fluorophenylalanine. Plant Sci. 1987;49(3):223–9.
[17]
Flick CE, Jensen RA, Evans DA. Isolation, Protoplast Culture, and Plant Regeneration of PFP-Resistant Variants of Nicotiana tabacum Sulsu. Z Pflanzenphysiol. 1981;103(3):239–45.