Biopolym. Cell. 1994; 10(2):5-10.
Some new data concerning the mutagenic action of DNA
1Gershenson S. M., 1Alexandrov Yu. N.
  1. Institute of Plant Physiology and Genetics, NAS of Ukraine
    31/17, Vasylkivska, Kyiv, Ukraine, 03022

Abstract

It is shown that spermatozoa of Drosophila melanogaster can transfer molecules of exogenous DNA (or their fragments) from a solution of this DNA injected into the haemocel of an adult male into the ooplasm of the egg. As a result, mutations induced in the descendants arise both in the paternal and the maternal chromosomes obtained by them. The mutagenic effect of exogenous DNA has a prolonged character inducing mutations in the descendants during many cell generations after DNA-treatment of their father. The mechanism of such a prolongation of the mutagenic action of DNA is discussed.

References

[1] Gershenson SM, Alexandrov YuN, Malyuta SS. Mutagenic Effect of DNA and viruses in Drosophila. Kiev: Naukova Dumka, 1975; 160 p.
[2] Gershenson SM. Mutagenic action of DNA, insertion, transposition and gene instability. Proc. XIV Int. Congr. of Genet. Moscow: Mir, 1980. V. 1, book 2: 91-115.
[3] Brackett BG, Baranska W, Sawicki W, Koprowski H. Uptake of heterologous genome by mammalian spermatozoa and its transfer to ova through fertilization. Proc Natl Acad Sci U S A. 1971;68(2):353-7.
[4] Lavitrano M, Camaioni A, Fazio VM, Dolci S, Farace MG, Spadafora C. Sperm cells as vectors for introducing foreign DNA into eggs: genetic transformation of mice. Cell. 1989;57(5):717-23.
[5] Siracuso G, Camaioni A, Russ MA et al. Sperm as carrier of foreign DNA. Abs.tr. 12 Int. Meet. ESHRE and ESCO. 1990. Suppl. 34 p.
[6] Fahmy OG, Fahmy MJ. Genetic properties of exogenous deoxyribonucleic acid at various levels of degradation in Drosophila melanogaster. Nature. 1965;207(996):507-10.
[7] Gershenson SM, Zilberman RA, Levochkina OA et al. Evoke mutations in Drosophila melanogaster Timo nucleic acid. Zh Obshch Biol. 1948. 9, No 2: 69-88.
[8] Gershenson SM. Delayed mutagenic effect of DNA in Drosophila. Mechanism of mutation and inducing factors. Praha : Academia, 1965: 291-293.
[9] Mathew C. The production of recessive lethals by calf-thymus dna in Drosophila. Genet Res. 1965;124:163-74.
[10] Khan AH, Alderson T. Mutagenic effect of irradiated and unirradiated DNA in Drosophila. Nature. 1965;208(5011):700–2.
[11] Fahmy OG, Fahmy DJ. Induction of mutations by deoxyribonucleic acid in Drosophila melanogaster. Nature. 1961;191:776-9.
[12] Gerasimova TI. Transposition of mobile elements and their role in insertion mutagenesis in Drosophila. Stability and variability of the genome. Moscow: Nauka. 1985; 26-39.
[13] Green MM. Mobile DNA element and spontaneous gene mutations. Eukaryotic transposable elements as mutagenic agents. New York : Cold. Spring Harbor Lab., 1988: 41-50.
[14] Rubin GM. Dispersed repetitive DNA in Drosophila. Mobile Genetic Elements. New York: Acad, press, 1983: 329-361.
[15] Terracol R. Transcription of rDNA insertions in bobbed mutants of Drosophila melanogaster. Genet Res. 1986;48(3):167-74.
[16] Flavell AJ, Ish-Horowicz D. The origin of extrachromosomal circular copia elements. Cell. 1983;34(2):415-9.
[17] Shiba T, Saigo K. Retrovirus-like particles containing RNA homologous to the transposable element copia in Drosophila melanogaster. Nature. 1983;302(5904):119-24.
[18] Emori Y, Shiba T, Kanaya S, Inouye S, Yuki S, Saigo K. The nucleotide sequences of copia and copia-related RNA in Drosophila virus-like particles. Nature. 1985 Jun 27-Jul 3;315(6022):773-6.