Biopolym. Cell. 1994; 10(1):100-103.
Chickens cartilage type II collagens isoelectrical focusing with different ration's providing with Ca and vitamin D analogs
- Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine
1, Murmans'ka Str., Kyiv, Ukraine, 02094
Abstract
Influence of rickets, Ca from diet and different doses of vitamin D analogs on cartilage type II collagen surface charge was studied in experiments on chickens. Chicken cartilage collagen pi were established. It was shown that rachitic type II collagen molecule had more negative charge then normal one. This effect wasn't compensated with Ca load at the absence of vitamin D3. Influences of vitamin D3 hydroxy-analogs and maximal dose of 3β-fluorovitamin D3 were analogous to its effect.
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References
[1]
Bauman VK. Biochemistry and physiology of vitamin D. Zinatne, Riga, 1989. 480 p.
[2]
Bondarenko LB, Yakhimovich RI, Gogoman IV, Bauman VK, Valinietse MJ. Influence of 3b-fluoroVitamin D3 and 1a,25-dihydroxyvitamin D3 on chicken bone and cartilage collagens. Doklady Akad Nauk Ukr SSR. 1991; (7):138-43.
[3]
Trelstad RL, Catanese VM, Rubin DF. Collagen fractionation: separation of native types I, II and III by differential precipitation. Anal Biochem. 1976;71(1):114-8.
[4]
Troitskiy GV, Azhitskiy BYu. Isoelectric focusing of proteins in self-organizing and artificial pH gradients. Kiev: Naukova Dumka, 1984. 215 p.