Biopolym. Cell. 1994; 10(1):31-36.
The restriction analysis of thymus and spleen DNA of a rat γ-irradiated at dose 5 Gy
1Lichina M. V., 1Kostuk G. V., 1Mitrochin Yu. I., 1Shugalii A. V.
  1. Institute of Chemical Physics, RAS in Chernogolovka
    Academician Semenov avenue 1, Chernogolovka, Moscow region, Russian Federation, 142432


1,5–3 hours after a single γ-irradiation of a rat the common pattern of EcoRI-restriction is unchanged but the parts some restriction fragments varied. The concentration of a fragments 2300 and 1500 base pairs rises in 2–3,5 times, fragment 345 base pair declines in 5–10 times. In a thymus the variations are occured after 3 hours, in a spleen – 1,5 hours after γ-irradiation with a future tendency to normalization.


[1] Komar VYe, Khanson KP. Information macromolecule in radiation injury of cells. M. : Atomizdat, 1980. 175 p.
[2] Koggl J. Biological effects of radiation. Ed. AN Dedenkova. Energoatomizdat, 1986. 184 p.
[3] George AM, Cramp WA. The effects of ionizing radiation on structure and function of DNA. Prog Biophys Mol Biol. 1987;50(3):121-69.
[4] Moskalyova EY, Ilyushina NA. DNA damage by the ionizing radiation and repair. Itogi nauki i tekhniki. VINITI 1990: (ser Radibiology; vol. 9) 5-113.
[5] Synzynys B. I., Sayenko A. S, Pelevina I. I.. Itogi nauki i tekhniki. M. : VINITI 1990: 114-213
[6] Stark GR, Wahl GM. Gene amplification. Annu Rev Biochem. 1984;53:447-91.
[7] Kreiss M., Baumstark-Khan C., Rink H. Int J Radiat Biol. 1986;49(4):702.
[8] Lapeyre JN, Becker FF. Analysis of highly repeated DNA sequences of rat with EcoR1 endonuclease. Biochim Biophys Acta. 1980;607(1):23-35.
[9] Spitkovskii DD, Zborovskaia IB, Kiselev FL. Transcription of cellular oncogenes in human tumors. Mol Biol (Mosk). 1986;20(5):1409-21.
[10] Lomholt B, Frederiksen S. Detection of a few picograms of DNA on polyacrylamide gels by silver staining. Anal Biochem. 1987;164(1):146-9.
[11] Lichina M. V., Shugaliy A. V., Todorov I. N. VI Conf. spectroscopy for biopolymers. Kharkiv, 1988: 194-195.
[12] Voigt JM, Topal MD. O6-methylguanine in place of guanine causes asymmetric single-strand cleavage of DNA by some restriction enzymes. Biochemistry. 1990;29(6):1632-7.
[13] Richardson FC, Richardson KK. Alterations in DNA-restriction enzyme interactions by O4-alkyldeoxythymidines. Mol Carcinog. 1991;4(2):162-8.
[14] Pech M, Igo-Kemenes T, Zachau HG. Nucleotide sequence of a highly repetitive component of rat DNA. Nucleic Acids Res. 1979;7(2):417-32.
[15] Asano S, Hibino Y, Ikeda Y, Iwakami N, Sugano N. Affinity of a DNA with highly repetitive sequence for nuclear proteins from rat liver. Biochem Int. 1989;19(4):871-80.
[16] Singh SP, Lavin MF. DNA-binding protein activated by gamma radiation in human cells. Mol Cell Biol. 1990;10(10):5279-85.
[17] Azorin F, Hahn R, Rich A. Restriction endonucleases can be used to study B-Z junctions in supercoiled DNA. Proc Natl Acad Sci U S A. 1984;81(18):5714-8.
[18] Varshavsky A. On the possibility of metabolic control of replicon "misfiring": relationship to emergence of malignant phenotypes in mammalian cell lineages. Proc Natl Acad Sci U S A. 1981;78(6):3673-7.
[19] Stark GR, Debatisse M, Giulotto E, Wahl GM. Recent progress in understanding mechanisms of mammalian DNA amplification. Cell. 1989;57(6):901-8.
[20] Schimke RT, Sherwood SW, Hill AB, Johnston RN. Overreplication and recombination of DNA in higher eukaryotes: potential consequences and biological implications. Proc Natl Acad Sci U S A. 1986;83(7):2157-61.
[21] Schimke RT. Gene amplification in cultured cells. J Biol Chem. 1988;263(13):5989-92.