Biopolym. Cell. 1985; 1(5):259-265.
http://dx.doi.org/10.7124/bc.00018C
Reviews
Radiation-induced unscheduled synthesis of DNA and DNA polymerase activity at the nuclear matrix
1Bezlepkin V. G., 1Malinovsky Yu. Yu., 1Gaziev A. I.
  1. Institute of Biological Physics, Academy of Sciences of the USSR
    Pushchino, Moscow Region, USSR

Abstract

At the nuclear matrix of UV- and Y-irradiated cells from Zajdela hepatoma the higher level of hydroxyurea-resistant DNA synthesis is observed in the initial (1.5-5 min) period of postradiation incubation in comparison with total nuclear DNA. But 1-β-D arabinofuranosylcytosine-resistant radiation-induced DNA synthesis is the same both in the nuclear matrix and in the intact nuclei of these cells. Both DNA polymerase α and DNA polymerase β are shown to participate in DNA synthesis at the nuclear matrix. DNA polymerase β is more strongly associated with the nuclear matrix of normal liver cells in comparison with nuclei of the regenerating liver. The poly-(ADP-ribose)synthetase activity is shown to be associated with the nuclear matrix. Under the activity inhibition the hydroxyurea-resistant DNA synthesis is, mainly, induced in the nuclear matrix.
Full text: (PDF, in Russian)

References

[1] Berezney R, Coffey DS. Identification of a nuclear protein matrix. Biochem Biophys Res Commun. 1974;60(4):1410-7.
[2] Paulson JR, Laemmli UK. The structure of histone-depleted metaphase chromosomes. Cell. 1977;12(3):817-28.
[3] Cook PR, Brazell IA. Supercoils in human DNA. J Cell Sci. 1975;19(2):261-79.
[4] Smith HC, Berezney R. Nuclear matrix-bound deoxyribonucleic acid synthesis: an in vitro system. Biochemistry. 1982;21(26):6751-61.
[5] Aelen JM, Opstelten RJ, Wanka F. Organization of DNA replication in Physarum polycephalum. Attachment of origins of replicons and replication forks to the nuclear matrix. Nucleic Acids Res. 1983;11(4):1181-95.
[6] Nelkin BD, Pardoll DM, Vogelstein B. Localization of SV40 genes within supercoiled loop domains. Nucleic Acids Res. 1980;8(23):5623-33.
[7] van Eekelen CA, van Venrooij WJ. hnRNA and its attachment to a nuclear protein matrix. J Cell Biol. 1981;88(3):554-63.
[8] Barrack ER, Coffey DS. The specific binding of estrogens and androgens to the nuclear matrix of sex hormone responsive tissues. J Biol Chem. 1980;255(15):7265-75.
[9] Hemminki K, Vainio H. Preferential binding of benzo[a]pyrene into nuclear matrix fraction. Cancer Lett. 1979;6(3):167-73.
[10] Tan Eng M. Autoimmunity to nuclear antigens. In: The Cell Nucleus: Chromatin. Ed. H. Busch. New York: Acad, press, 1979; 7)part D):413-456.
[11] Berger NA, Weber G, Kaichi AS. Characterization and comparison of poly(adenosine dephosphoribose) synthesis and DNA synthesis in nucleotide-permeable cells. Biochim Biophys Acta. 1978;519(1):87-104.
[12] Skidmore CJ, Davies MI, Goodwin PM, Halldorsson H, Lewis PJ, Shall S, Zia'ee AA. The involvement of poly(ADP-ribose) polymerase in the degradation of NAD caused by gamma-radiation and N-methyl-N-nitrosourea. Eur J Biochem. 1979;101(1):135-42.
[13] Sims JL, Sikorski GW, Catino DM, Berger SJ, Berger NA. Poly(adenosinediphosphoribose) polymerase inhibitors stimulate unscheduled deoxyribonucleic acid synthesis in normal human lymphocytes. Biochemistry. 1982;21(8):1813-21.
[14] Hudack ED, Baker N. Studies on Ehrlich ascites cell nuclei. I. Methods of isolation of nuclei. Exp Cell Res. 1961;22:327-37.
[15] Berezney R, Buchholtz LA. Dynamic association of replicating DNA fragments with the nuclear matrix of regenerating liver. Exp Cell Res. 1981;132(1):1-13.
[16] Bollum FJ. Mammalian DNA polymerases. Prog Nucleic Acid Res Mol Biol. 1975;15(0):109-44.
[17] Edenberg HJ, Anderson S, DePamphilis ML. Involvement of DNA polymerase alpha in simian virus 40 DNA replication. J Biol Chem. 1978;253(9):3273-80.
[18] Downes CS, Collins AR, Johnson RT. International Workshop on inhibition of DNA repair. King's College, Cambridge, U.K., 29th June-2nd July 1982. Mutat Res. 1983;112(2):75-83.
[19] Markwell MA, Haas SM, Bieber LL, Tolbert NE. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978;87(1):206-10.
[20] Spirin AS. Spectrophotometric determination of total nucleic acids. Biokhimiia. 1958;23(5):656-62.
[21] McCready SJ, Cook PR. Lesions induced in DNA by ultraviolet light are repaired at the nuclear cage. J Cell Sci. 1984;70:189-96.
[22] Mullenders LH, Van Zeeland AA, Natarajan AT. Analysis of the distribution of DNA repair patches in the DNA-nuclear matrix complex from human cells. Biochim Biophys Acta. 1983;740(4):428-35.
[23] Falaschi A., Spadari S. The three polymerases of animal cells: properties and functions. In: DNA synthesis: Present and future . Eds I. Molineux, M. Kohijama. New York; London : Plenum press, 1978, p. 487-515.
[24] Chanpu S, M?ller M, Werner D. DNA polymerases in isolated 'nuclear matrix' of Ehrlich ascites cells. Biochem Biophys Res Commun. 1984;118(2):609-15.
[25] Seki S, Oda T, Ohashi M. Differential effects of aphidicolin on replicative DNA synthesis and unscheduled DNA synthesis in permeable mouse sarcoma cells. Biochim Biophys Acta. 1980;610(2):413-20.