Correction of DNA polymerase mistakes and 3\'→5\'-exonucleases of the rat liver

Belyakova, N. V.; Kleiner, N. E.; Kravetskaya, T. P.; Legina, O. K.; Timchenko, N. A.; Krutyakov, V. M.

doi:10.7124/bc.00018B

Biopolym. Cell. 1985; 1(5):253-258.

http://dx.doi.org/10.7124/bc.00018B

Reviews

Correction of DNA polymerase mistakes and 3'→5'-exonucleases of the rat liver

1Belyakova N. V., 1Kleiner N. E., 1Kravetskaya T. P., 1Legina O. K., 1Timchenko N. A., 1Krutyakov V. M.

B. P. Konstantinov Institute of Nuclear Physics, Academy of Sciences of the USSR
Gatchina, Leningrad distr., USSR

Abstract

One of the levels of correcting the DNA polymerase mistakes is the 3'→5'-exonuclease «proofreading» activity being absent or non-effective for mammalian DNA polymerases. Cells are supposed to contain correcting 3'→5'-exonuclease non-bound covalently with DNA polymerases. Three 3'→5'-exonucleases are isolated from the rat liver. To study their correcting ability both correct and incorrect [³H] dNMPs are attached to 3'-ends of poly[^d(A-T)] by means of DNA polymerase β or terminal transferase. The quality of templates obtained is checked by the measurement of correction specificity of the phage T4 DNA polymerase. 3'→5'-exonuclease, mainly able of removing non-complementary nucleotides from the DNA primer is established to arise under the liver regeneration.

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