Biopolym. Cell. 1985; 1(3):156-158.
http://dx.doi.org/10.7124/bc.000024
Short Communications
A study of lysine operon organization in Bacillus subtilis
1Aleksieva Z. M., 1Shevchenko T. N., 1Malyuta S. S.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

The pBR322 plasmid was used for constructing pLBl plasmid carrying genes of Bacillus subtills lysine biosynthesis. B. subtilis and E. coli strains (lysine-auxotrophs) are transformed. The plasmid is shown to complement both mutations in seven genes of lysine biosynthesis in E. coli cells and four mutations in B. subtills. The size of DNA insertion, according to the elctrophoresis data, is 4.5×106.
Full text: (PDF, in Russian)

References

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