Biopolym. Cell. 1985; 1(3):125-131.
http://dx.doi.org/10.7124/bc.00001E
Cell Biology
2',3'-dideoxy-3'-aminonucleoside-5'-triphosphates inhibit repair DNA synthesis in rat liver chromatin
1Kraevsky A. A., 1Kukhanova M. K., 1Alexandrova L. A., 2Belyakova N. V., 2Krutyakov V. M.
  1. Institute of Molecular Biology, Academy of Sciences of the USSR
    Moscow, USSR
  2. B. P. Konstantinov Institute of Nuclear Physics, Academy of Sciences of the USSR
    Gatchina, Leningrad distr., USSR

Abstract

2',3-dideoxy-3'-aminonucleoside-5'-triphosphates are shown to be strong inhibitors of repair DNA synthesis in γ-irradiated rat liver chromatin. The activity of these compounds is comparable with that of the most effective inhibitors of the DNA-polymerase p-cataly-zed repair DNA synthesis
Full text: (PDF, in Russian)

References

[1] Chidgeavadze ZG, Beabealashvilli RS, Atrazhev AM, Kukhanova MK, Azhayev AV, Krayevsky AA. 2',3'-Dideoxy-3' aminonucleoside 5'-triphosphates are the terminators of DNA synthesis catalyzed by DNA polymerases. Nucleic Acids Res. 1984;12(3):1671-86.
[2] Atkinson MR, Deutscher MP, Kornberg A, Russell AF, Moffatt JG. Enzymatic synthesis of deoxyribonucleic acid. XXXIV. Termination of chain growth by a 2',3'-dideoxyribonucleotide. Biochemistry. 1969;8(12):4897-904.
[3] Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977;74(12):5463-7.
[4] Smith AJ. DNA sequence analysis by primed synthesis. Methods Enzymol. 1980;65(1):560-80.
[5] Kukhanova MK, Kochetkova SV, Kraevskii AA, Terent'ev LL, Rasskazov VA. Inhibition of DNA biosynthesis in sea urchin embryos by 2',3'-dideoxy-3'-aminonucleosides. Biokhimiia. 1983;48(10):1747-51.
[6] Kukhanova M. K., Kraevsky A. A., Terentieva N. A., Rasskazov V. A. Inhibition of replicative DNA synthesis in nuclei of sea urchin (Strongylocentrotus intermwium) embryo by 2', 3'-dideoxy-3'-aminonucleoside 5'-triphosphates. Biopolym. Cell. 1985; 1(2):79-86.
[7] Cozzarelli NR. The mechanism of action of inhibitors of DNA synthesis. Annu Rev Biochem. 1977;46:641-68.
[8] Lynch WE, Surrey S, Lieberman I. Nuclear deoxyribonucleic acid polymerases of liver. J Biol Chem. 1975;250(20):8179-83.
[9] Beliakova NV, Naryzhnyi SN, Krutiakov VM. Mechanism of activating action of ATP on repair synthesis of DNA in chromatin. Mol Biol (Mosk). 1980;14(3):586-94.
[10] Miller MR, Lui LH. Participation of different DNA polymerases in mammalian DNA repair synthesis is not related to "patch size". Biochem Biophys Res Commun. 1982;108(4):1676-82.
[11] Haseltine WA, Gordon LK, Lindan CP, Grafstrom RH, Shaper NL, Grossman L. Cleavage of pyrimidine dimers in specific DNA sequences by a pyrimidine dimer DNA-glycosylase of M. luteus. Nature. 1980;285(5767):634-41.
[12] Painter RB. Repair in mammalian cells: overview. In: Mol. Mech. Repair DNA Ed. A. Kornberg. N. Y.; London : Acad. Press, 1975, part B, p. 595-600.
[13] Krutiakov VM, Kravetskaia TP. Endogenous DNA synthesis in isolated chromatin. Mol Biol (Mosk). 1978;12(3):654-62.
[14] Zaitseva V. E., Dyatkina N. B., Krayevsky A. A., Skaptsova N. V., Turina O. V., Gnuchev N. V., Gottikh B. P., Azhayev A. V. Aminonucleosides and their derivatives. XI. Synthesis of 3'-amino-2',3'-dideoxynucleoside 5'-triphosphates. Russian Journal of Bioorganic Chemistry. 1984, 10 (5):670-680.
[15] Schinazi RF, Chen MS, Prusoff WH. Antiviral and antineoplastic activities of pyrimidine arabinosyl nucleosides and their 5'-amino derivatives. J Med Chem. 1979;22(10):1273-7.
[16] Ono K, Ogasawara M, Matsukage A. Inhibition of the activity of DNA polymerase alpha by 2',3'-dideoxythymidine 5'-triphosphate. Biochem Biophys Res Commun. 1979;88(4):1255-62.
[17] Edenberg HJ, Anderson S, DePamphilis ML. Involvement of DNA polymerase alpha in simian virus 40 DNA replication. J Biol Chem. 1978;253(9):3273-80.
[18] Byars N, Kidson C. DNA chain termination by 2',3'-dideoxythymidine in replicating mammalian cells. Biochemistry. 1975;14(14):3159-64.