Biopolym. Cell. 1985; 1(3):121-124.
http://dx.doi.org/10.7124/bc.00001D
Structure and Function of Biopolymers
Some physicochemical properties of the protein of inclusion bodies of the Nuclear polyhedrosis and Granulosis viruses of Agrotis segetum
1Kozlov E. A., 1Levitina T. L., 1Gusak N. M., 1Serebryany S. B.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

Dodecylsulphate-polyacrylamide gel electrophoresis has shown that polyhedra and gra-nula of the nuclear polyhedrosis (NPV) and granulosis viruses (GV) of Agrotis sege-ium consist of protein with molecular weight of 28000 and 27500, respectively. Using the dansylation method it was shown that glycine and methionine are N-terminal amino acids of the polyhedral (polyhedrin) and granule (granulin) proteins, respectively. Ami-no acid composition is determined and fingerprints are compiled for the both proteins. According to physicochemical properties granulin of GV and polyhedrin of NPV of the baculoviruses, affecting the same host, differ to a greater extent from polyhedrins of NPV affecting various hosts.
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