Вибіркове введення спін-мітки у залишок N-ацетилнейрамінової кислоти олігосахаридного угруповання Fab-області імуноглобуліну М
DOI:
https://doi.org/10.7124/bc.000214Анотація
Запропоновано методику введення спін-мітки (2,2,6,6-тетраметил-4-амінопіперидин-1-оксил) у залишок N-ацетилнейрамінової кислоти олігосахаридного угруповання Fab-області IgM.Посилання
Timofeev VP. Segmental mobility of poly (s) and the spin-label method. Mol biol (Mosk). 1986; 20(3):697-711.
Timofeev VP, Lapuk VA. Study of the irreversible conformation change of immunoglobulin M by spin-labeling at the carbohydrate and peptide moieties of the molecule. Mol Biol (Mosk). 1982;16(2):403-10.
Lapuk VA, Timofeev VP, Tchukchrova AI, Khatiashvili NM, Kiseleva TM. Some peculiarities of the dynamics of the immunoglobulin M structure. J Biomol Struct Dyn. 1984;2(1):63-76.
Sharom FJ, Ross TE. Spin labelling of sialic acid and galactose residues on lymphocyte plasma membrane: effects of lectins on oligosaccharide dynamics. Mol Immunol. 1985;22(5):521-30.
Putnam FW, Florent G, Paul C, Shinoda T, Shimizu A. Complete amino acid sequence of the Mu heavy chain of a human IgM immunoglobulin. Science. 1973;182(4109):287-91.
Kaverzneva ED, Chukhrova AI, Vikha GV. The role of carbohydrate groups in immunoglobulins M. V. Steric accessibility of oligosaccharide groupings in macroimmunoglobulins. Russian Journal of Bioorganic Chemistry. 1978; 4(3):369-374.
Lapuk VA, Shmakova FV, Kaverzneva ED. Role of carbohydrate groups in immunoglobolins M. I.Isolation and properties of human immunoglobulin M with carbohydrate groups bound to L-chains. Russian Journal of Bioorganic Chemistry. 1975; 1(8):1134-9
Warren L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959;234(8):1971-5.
Dudich IV, Timofeev VP, Vol'kenshtein MV, Misharin AIu. Macromolecule rotative correlation time measurement by ESR for covalently bound spin label. Mol Biol (Mosk). 1977;11(3):685-93.
Timofeev VP, Dudich IV, Sykulev YK, Nezlin RS. Rotational correlation times of IgG and its fragments spin-labeled at carbohydrate or protein moieties. Spatially fixed position of the Fc carbohydrate. FEBS Lett. 1978;89(2):191-5.
Timofeev VP, Dudich IV, Volkenstein MV. Comparative study of dynamic structure of pig and chicken aspartate aminotransferases by measuring the rotational correlation time. Biophys Struct Mech. 1980;7(1):41-9.
Timofeev VP. Characterization of dynamic behavior of side groups of globular proteins by spin-label, NMR and luminescence polarization methods. Mol Biol (Mosk). 1983;17(3):519-31.