Виявлення і дослідження субгеномної РНК Х-вірусу картоплі
DOI:
https://doi.org/10.7124/bc.0001CCАнотація
В інфікованих Х-вірусом картоплі (ХВК) рослинах дурману виявлено вірусоспецифічну, низькомолекулярну РНК (0,3·106), яка гибрідизується з кДНК, синтезованою на РНК ХВК. Вона виявляється в інфікованих рослинах на третій день після зараження, седиментує в лінійному градієнті сахарози в основному між 18S і 5S рибосомними РНК, має послідовність нуклеотидів, що відповідає 3'-кінцю геномної РНК, і транслюється у безклітинній білоксинтезувальній системі в білок з ідентичною білку оболонки ХВК електрофоретичною рухливістю, тобто є субгеномною РНК ХВК для синтезу вірусного білка оболонки.Посилання
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